FastQCFastQC Report
Thu 26 May 2016
ERR580061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6858504
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173720.25329138832608394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142930.20839821628740027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83890.12231530374553984No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101500.051.097561
GTATCAA183700.041.7991751
CAACGCA217900.033.888165
ATCAACG219950.033.5919653
TATCAAC234250.032.368682
AACGCAG239300.031.2548246
TCAACGC239750.031.1521434
ACGCAGA260200.028.2753187
GTACTAG37100.028.0097391
CGCAGAG268400.027.2895348
GTACATG252400.026.5843051
GTGGTAT38100.026.0404321
TACATGG259300.025.4164982
ACATGGG257050.024.8672713
CTAACGC15000.024.4405043
GCAGAGT296800.024.222229
TAACGCC15650.023.7265954
TCTAACG15850.023.429772
GCATCGT37450.022.7069768
CTGTGCG47700.022.3611379
Produced by FastQC (version 0.11.5)