FastQCFastQC Report
Thu 26 May 2016
ERR580061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6858504
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153930.22443669931518592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135880.19811900671050128No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79210.11549165823917286No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104250.053.6694561
GTATCAA184350.039.3276061
CAACGCA221700.031.6091695
ATCAACG223400.031.5165943
TATCAAC237000.030.3720172
AACGCAG242900.029.2737086
TCAACGC242250.029.1802044
ACGCAGA267900.026.138457
GTACTAG36750.026.0993181
GTGGTAT38900.025.382011
GTACATG252100.025.3456211
CGCAGAG276250.025.2461268
TACATGG253100.024.706222
TAGTACT53800.024.2866044
CTGTGCG51850.023.8374969
CTAGTAC56150.023.6890323
ACATGGG258750.023.5415633
GCAGAGT303900.022.5774969
TAACGCC15450.022.511664
CTAACGC15900.022.466073
Produced by FastQC (version 0.11.5)