FastQCFastQC Report
Thu 26 May 2016
ERR580060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5708384
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90570.15866136545824527No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76190.13347034817559578No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76200.047.1475451
GTATCAA135900.039.7923051
CAACGCA156150.033.0621835
ATCAACG159100.032.4511453
TATCAAC168500.031.674452
TCAACGC173650.029.8940334
AACGCAG175200.029.7744056
ACGCAGA193600.026.6038327
CGCAGAG199550.025.7161588
GTACTAG28400.024.8366871
GTACATG221350.022.6251141
GCAGAGT221650.022.4553839
CTAACGC12600.022.3912623
GTGGTAT38250.022.2519381
TACATGG224750.021.9476932
GCATCGT28900.021.6266378
ACATGGG225150.021.1143173
TAGGCAT39550.020.6858775
GTATAAG37300.020.5494161
GTGATCG20950.020.4123278
Produced by FastQC (version 0.11.5)