FastQCFastQC Report
Thu 26 May 2016
ERR580060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5708384
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83080.14554031403633672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73550.12884557170645844No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80300.045.7609521
GTATCAA140200.039.0959051
CAACGCA156700.033.17075
ATCAACG159550.032.490673
TATCAAC172200.031.2769092
AACGCAG174500.030.3241526
TCAACGC177350.029.4949554
GTACTAG28850.028.7766341
ACGCAGA194400.026.7609127
CGCAGAG198400.026.1266298
GTACATG220400.022.7935091
GCAGAGT223050.022.6491539
TACATGG221250.021.7514922
GTATAAG35950.021.387591
GTGGTAT36500.021.194551
ACATGGG219100.021.0000483
TAGGCAT42600.020.9610275
GAGTACT98650.019.72182312-13
GCATCGT34750.019.609338
CAGAGTA218950.019.08047710-11
Produced by FastQC (version 0.11.5)