##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580060_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5708384
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.470053696457704	34.0	31.0	34.0	31.0	34.0
2	32.68359276460728	34.0	31.0	34.0	31.0	34.0
3	32.76331655333629	34.0	31.0	34.0	31.0	34.0
4	36.24808860090702	37.0	37.0	37.0	35.0	37.0
5	36.17552340557327	37.0	35.0	37.0	35.0	37.0
6	36.20180930364881	37.0	37.0	37.0	35.0	37.0
7	36.172967165488515	37.0	37.0	37.0	35.0	37.0
8	36.16242722984298	37.0	37.0	37.0	35.0	37.0
9	37.93953823008403	39.0	38.0	39.0	35.0	39.0
10-11	37.88644220500933	39.0	38.0	39.0	35.0	39.0
12-13	37.85382920980789	39.0	38.0	39.0	35.0	39.0
14-15	39.24150460095186	41.0	39.0	41.0	36.0	41.0
16-17	39.225343634906125	41.0	39.0	41.0	36.0	41.0
18-19	39.220214950500875	41.0	39.0	41.0	36.0	41.0
20-21	39.20070662730468	41.0	39.0	41.0	36.0	41.0
22-23	39.12827202935191	40.0	39.0	41.0	35.5	41.0
24-25	39.04745537441069	40.0	38.5	41.0	35.0	41.0
26-27	38.90829313514998	40.0	38.0	41.0	35.0	41.0
28-29	38.802590277738844	40.0	38.0	41.0	35.0	41.0
30-31	38.70126615518508	40.0	38.0	41.0	35.0	41.0
32-33	38.569594477176025	40.0	38.0	41.0	34.0	41.0
34-35	38.54989214110333	40.0	38.0	41.0	34.5	41.0
36-37	38.60561947128995	40.0	38.0	41.0	35.0	41.0
38-39	38.518410201556165	40.0	38.0	41.0	34.0	41.0
40-41	38.39942968097451	40.0	38.0	41.0	34.0	41.0
42-43	38.384000095298425	40.0	38.0	41.0	34.0	41.0
44-45	38.30506794917791	40.0	38.0	41.0	34.0	41.0
46-47	38.13052984872777	40.0	38.0	41.0	33.0	41.0
48-49	37.9322174191505	40.0	37.0	41.0	33.0	41.0
50-51	37.763794709676155	40.0	37.0	41.0	33.0	41.0
52-53	37.588252822515095	40.0	36.5	41.0	33.0	41.0
54-55	37.38382500196202	39.5	36.0	41.0	32.5	41.0
56-57	37.16724417978889	39.0	35.5	41.0	32.0	41.0
58-59	36.93066137456765	39.0	35.0	41.0	32.0	41.0
60-61	36.66744117074114	38.5	35.0	40.0	31.0	41.0
62-63	36.37938801944648	38.0	35.0	40.0	31.0	41.0
64-65	36.084912209830314	37.0	35.0	40.0	31.0	41.0
66-67	35.75555717695236	37.0	35.0	39.5	30.5	41.0
68-69	35.397228182266645	36.0	34.5	39.0	30.0	41.0
70-71	35.06709271835952	36.0	34.0	39.0	30.0	40.5
72-73	34.70854247016318	35.0	34.0	38.0	29.5	40.0
74-75	34.35082354305527	35.0	34.0	37.0	29.0	39.0
76-77	33.12171054014586	34.5	32.5	36.0	27.5	39.0
78-79	33.76758781469502	35.0	33.5	36.0	29.0	39.0
80-81	33.70874226401027	35.0	34.0	36.0	29.0	37.5
82-83	33.504863110120134	35.0	34.0	35.5	29.0	37.0
84-85	33.288737845947296	35.0	34.0	35.0	29.0	37.0
86-87	33.11553742705466	35.0	33.0	35.0	29.0	36.0
88-89	32.95892252518401	35.0	33.0	35.0	29.0	36.0
90-91	32.79867279426192	35.0	33.0	35.0	29.0	36.0
92-93	32.68241151961746	35.0	33.0	35.0	28.5	35.0
94-95	32.58526160818894	35.0	33.0	35.0	27.0	35.0
96-97	32.48663387046142	35.0	33.0	35.0	27.0	35.0
98-99	32.411525486021965	35.0	33.0	35.0	27.0	35.0
100	31.092879525974425	34.0	30.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.17176039167438262
1101	2	0.08891830080752783
1101	3	0.061159013788127936
1101	4	0.022822896254211855
1101	5	0.019813369743609144
1101	6	0.019164192254812917
1101	7	-0.02281482725352646
1101	8	0.027904877314888665
1101	9	0.03691493177127825
1101	10-11	0.0043618700374921104
1101	12-13	-0.03269459197878888
1101	14-15	0.03660380135933394
1101	16-17	-0.00871173895867372
1101	18-19	-0.035996469790127605
1101	20-21	-0.03946913789891937
1101	22-23	-0.01719112850645388
1101	24-25	-0.07584363031509866
1101	26-27	-0.026233353067432574
1101	28-29	0.011929070839052258
1101	30-31	-0.05682207667783956
1101	32-33	-0.027666630989969576
1101	34-35	-0.128962393232392
1101	36-37	0.014398712447245998
1101	38-39	0.023885074319942134
1101	40-41	0.04359829114012115
1101	42-43	-0.03689624803136127
1101	44-45	-0.05919694050567159
1101	46-47	0.010867067215706072
1101	48-49	-0.051584466997937284
1101	50-51	-0.06752897470200026
1101	52-53	-0.051670295635844354
1101	54-55	-0.05336735733646236
1101	56-57	-0.059773332087978304
1101	58-59	-0.17815787002059125
1101	60-61	-0.1540419442326737
1101	62-63	-0.07888716816456309
1101	64-65	-0.1242719843148592
1101	66-67	-0.13837124540609125
1101	68-69	-0.19263226115886312
1101	70-71	-0.2764761493882162
1101	72-73	-0.1364889147502737
1101	74-75	-0.0899030974793007
1101	76-77	-0.05487703023081991
1101	78-79	-0.048992069850605446
1101	80-81	-0.06789065244051073
1101	82-83	-0.043896113545926596
1101	84-85	-0.050507629609249705
1101	86-87	0.005542133599853116
1101	88-89	0.047820548780173056
1101	90-91	-0.02408823152109818
1101	92-93	-0.13417539465773132
1101	94-95	-0.06189062197483253
1101	96-97	-0.04073173459944002
1101	98-99	-0.1268168204848692
1101	100	-0.054953756244565
1102	1	0.10600451459357885
1102	2	0.015352240535335682
1102	3	-0.01428887939696466
1102	4	-0.007352912809132306
1102	5	0.009132005556111267
1102	6	0.03686090091251515
1102	7	0.008158409974519998
1102	8	0.006215096201451331
1102	9	-0.021406622855934643
1102	10-11	0.02420199056825112
1102	12-13	-0.008218208320933229
1102	14-15	0.006587664609739363
1102	16-17	-0.0548013947069137
1102	18-19	-0.05793866142707316
1102	20-21	-0.08089999332850795
1102	22-23	-0.11931942826669228
1102	24-25	-0.08036743330794849
1102	26-27	-0.10069182289480239
1102	28-29	-0.10433935196009969
1102	30-31	-0.10924414127789106
1102	32-33	-0.20253495853975778
1102	34-35	-0.14368858863619494
1102	36-37	-0.05753429720363101
1102	38-39	-0.11314452804782604
1102	40-41	-0.11343545493947005
1102	42-43	-0.016036233016812673
1102	44-45	-0.07628799036814371
1102	46-47	-0.0983953752636495
1102	48-49	-0.1420965080759018
1102	50-51	-0.07358157310468982
1102	52-53	-0.10943352408656892
1102	54-55	-0.10308604290241874
1102	56-57	-0.1657359955040718
1102	58-59	-0.14838304833012472
1102	60-61	-0.13417784123954846
1102	62-63	-0.08058519575231315
1102	64-65	-0.10712169340443012
1102	66-67	-0.14688626910145786
1102	68-69	-0.1295337402700767
1102	70-71	-0.15802033934088655
1102	72-73	-0.11795837516956453
1102	74-75	-0.15005154967406753
1102	76-77	-0.12844778763311382
1102	78-79	-0.15064446903475925
1102	80-81	-0.1276982314947901
1102	82-83	-0.07892577426650149
1102	84-85	-0.044419486577780276
1102	86-87	-0.08814205106371986
1102	88-89	-0.017165420500774076
1102	90-91	-0.11046813799340072
1102	92-93	-0.07670338827348644
1102	94-95	-0.15060887121955346
1102	96-97	-0.07650125353111292
1102	98-99	-0.14045230313561063
1102	100	-0.13698539990678427
1103	1	0.10572009328180343
1103	2	0.0561810104213194
1103	3	0.05302325859049262
1103	4	0.026339478778865555
1103	5	0.0052586220738461975
1103	6	0.06432682085347352
1103	7	0.04244470466259287
1103	8	0.04992225807198736
1103	9	0.010839628270389312
1103	10-11	0.059100334556866585
1103	12-13	0.020890833465777803
1103	14-15	0.05329305585193822
1103	16-17	0.0818592412416308
1103	18-19	0.04962766708720778
1103	20-21	0.01999419466691421
1103	22-23	-0.03781001704628295
1103	24-25	-0.07377879248523556
1103	26-27	-0.014276702623085669
1103	28-29	-0.050857789028157185
1103	30-31	-0.0471678312814916
1103	32-33	-0.02723443436215689
1103	34-35	-0.1273894811605416
1103	36-37	-0.045466817251039515
1103	38-39	-0.050917145648718076
1103	40-41	-0.05852376911547452
1103	42-43	-0.06541720754385949
1103	44-45	-0.02435413831113209
1103	46-47	-0.03877706020991667
1103	48-49	-0.02634157828011041
1103	50-51	-0.046270786571369626
1103	52-53	-0.04334598289040059
1103	54-55	0.011429978456483525
1103	56-57	-0.02906714696563739
1103	58-59	-0.03609115935866214
1103	60-61	0.02768717270781451
1103	62-63	0.019725505847127067
1103	64-65	-0.10776467578712356
1103	66-67	-0.10574782117043924
1103	68-69	-0.11873770208382695
1103	70-71	-0.09778416276765967
1103	72-73	-0.10970404297555092
1103	74-75	-0.17200350052799251
1103	76-77	-0.19966751816293282
1103	78-79	-0.1385908025375855
1103	80-81	-0.0405783532560946
1103	82-83	0.013348530704583084
1103	84-85	-0.025089616401011483
1103	86-87	-0.012528147136954715
1103	88-89	-0.04348962438302806
1103	90-91	-0.048075039419167354
1103	92-93	-0.09135855868306919
1103	94-95	-0.05249293897933427
1103	96-97	-0.011075022078166796
1103	98-99	-0.028242766513180584
1103	100	-0.04639524953758567
1104	1	0.12607716733398178
1104	2	0.09415717957546832
1104	3	0.060759571874946516
1104	4	0.02570536107126742
1104	5	-0.019964804733660912
1104	6	0.005723880035297668
1104	7	0.02434245952579772
1104	8	-0.023474861009624703
1104	9	0.002687106530864014
1104	10-11	0.014735344818866736
1104	12-13	0.007974971157800326
1104	14-15	0.061523616676787185
1104	16-17	-4.0243752364688135E-4
1104	18-19	-0.05506028769510607
1104	20-21	-0.06689082079471831
1104	22-23	-0.0794374883731237
1104	24-25	-0.08407507894789745
1104	26-27	-0.026871227520899765
1104	28-29	-0.09246749246121055
1104	30-31	-0.014876927599281942
1104	32-33	-0.1001702864420011
1104	34-35	-0.12198267839853827
1104	36-37	-0.043143278880172886
1104	38-39	0.006486313778033548
1104	40-41	-0.011643849889864555
1104	42-43	0.07734573915970344
1104	44-45	-0.050723000023786824
1104	46-47	-0.004605032769639195
1104	48-49	-0.03892611987973993
1104	50-51	-0.020023046629631835
1104	52-53	-0.05829763579774294
1104	54-55	-0.11208555561491806
1104	56-57	-0.02871477516574572
1104	58-59	0.0031164169425537125
1104	60-61	-0.0052423203676355
1104	62-63	0.013973283939328951
1104	64-65	0.01112174256559939
1104	66-67	0.0012054955010896151
1104	68-69	0.03887320195968158
1104	70-71	-0.03280951559109013
1104	72-73	-0.03157247843703459
1104	74-75	-0.07565350130645498
1104	76-77	-0.19491989534667908
1104	78-79	-0.0462296657584389
1104	80-81	0.015529802944016069
1104	82-83	0.03344835655545353
1104	84-85	-0.023756014152489513
1104	86-87	-0.055017331600133446
1104	88-89	-0.010218500466883995
1104	90-91	0.07395721721149329
1104	92-93	-0.0399167921527237
1104	94-95	0.03455374054597371
1104	96-97	-0.07642188880220857
1104	98-99	-0.005043111721370508
1104	100	0.01578266281927654
1105	1	0.16555553891841868
1105	2	0.10596785670306019
1105	3	0.06153986911858311
1105	4	0.08041686236082768
1105	5	0.02031802587892173
1105	6	0.05540284345505597
1105	7	0.035584109119689344
1105	8	0.04058468296528872
1105	9	0.023306796774456018
1105	10-11	0.02703591088162227
1105	12-13	0.036112711538372366
1105	14-15	0.06383071692648912
1105	16-17	0.004574481552346299
1105	18-19	0.017044430179289805
1105	20-21	0.015709799614540998
1105	22-23	-0.021678392336681895
1105	24-25	0.0096116568627167
1105	26-27	0.045016792996314337
1105	28-29	0.011196484582526978
1105	30-31	0.03290813710294316
1105	32-33	-0.004206039813446694
1105	34-35	-0.051144119738857796
1105	36-37	0.026530028114095217
1105	38-39	0.03045451220067008
1105	40-41	0.03171429732380915
1105	42-43	-0.01606908903249149
1105	44-45	0.04912942633296069
1105	46-47	0.059615297959673796
1105	48-49	0.046798315785018474
1105	50-51	0.027205129792946536
1105	52-53	-0.022006868914374422
1105	54-55	-0.01144781289986696
1105	56-57	-0.0018378830762415532
1105	58-59	0.027456846914937216
1105	60-61	0.004987860899440477
1105	62-63	0.01296572585299316
1105	64-65	0.07710115625830838
1105	66-67	0.03727273669686326
1105	68-69	0.11526599460441389
1105	70-71	0.04238896121266578
1105	72-73	0.04004608565729484
1105	74-75	-0.07983839241735069
1105	76-77	-0.04525935091047728
1105	78-79	0.04428656535801423
1105	80-81	0.09011670646309256
1105	82-83	0.0634506396690071
1105	84-85	0.08454935998866375
1105	86-87	0.04557115375711618
1105	88-89	0.09166882671215859
1105	90-91	0.0888741426932782
1105	92-93	0.08774859117691136
1105	94-95	0.0664995882674404
1105	96-97	-5.590212862855992E-4
1105	98-99	-0.01108128173816425
1105	100	0.1032571937304887
1106	1	0.11993019173959851
1106	2	0.06055657375573276
1106	3	-0.027192750792629283
1106	4	0.03403148142048451
1106	5	0.003968018406652618
1106	6	0.021537929029051384
1106	7	0.022682803304384436
1106	8	0.05019043351537533
1106	9	0.002308646514990187
1106	10-11	0.023801214711845375
1106	12-13	0.008662263530844427
1106	14-15	0.05868357067761565
1106	16-17	-0.005454609800203514
1106	18-19	0.030471321252314
1106	20-21	-0.004417995086818394
1106	22-23	-0.052201725337916116
1106	24-25	-0.004602672188077861
1106	26-27	-0.061000838048123285
1106	28-29	-0.028447165440546485
1106	30-31	0.0044852653149618504
1106	32-33	-0.012741087368134174
1106	34-35	0.016577396794609456
1106	36-37	0.017219379558213177
1106	38-39	0.0031749807358139037
1106	40-41	0.009858950229578056
1106	42-43	0.049310262862782395
1106	44-45	0.10729283394415035
1106	46-47	0.08243231862732614
1106	48-49	0.09584637026777187
1106	50-51	0.11246369988147364
1106	52-53	0.07484321469051736
1106	54-55	0.07049223290189133
1106	56-57	0.025000096852942022
1106	58-59	-0.02846164724775946
1106	60-61	-0.005288896424559653
1106	62-63	0.035306418359006386
1106	64-65	-0.019364739620478133
1106	66-67	-0.0561078497686367
1106	68-69	-0.06982319804210846
1106	70-71	-0.028586255271832783
1106	72-73	-0.014448182308825608
1106	74-75	-0.04450387182253479
1106	76-77	-0.05831462553772582
1106	78-79	-0.005714879751778312
1106	80-81	0.01319172035024252
1106	82-83	0.0998285765315714
1106	84-85	0.031441046307257636
1106	86-87	0.12400075764195151
1106	88-89	0.12415383751640974
1106	90-91	0.11468245252105902
1106	92-93	0.10333785995605638
1106	94-95	0.07760848798198339
1106	96-97	0.05221568351185368
1106	98-99	0.11340450002628444
1106	100	0.17317066572543283
1107	1	0.07251978305782103
1107	2	0.059347301993135204
1107	3	0.0248238965693659
1107	4	0.012172973630377726
1107	5	0.007142635549449494
1107	6	0.051655594794006277
1107	7	0.03044726467395975
1107	8	-0.016275905386791578
1107	9	-0.04248591259987933
1107	10-11	0.024135191427014036
1107	12-13	0.024155278197788732
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2316	12-13	0.03827954394048305
2316	14-15	0.06205492341885588
2316	16-17	0.04953900749869433
2316	18-19	0.06860261119125965
2316	20-21	0.07762264682263975
2316	22-23	0.07275521170515731
2316	24-25	0.11129779908384307
2316	26-27	0.12394926815676399
2316	28-29	0.10943685192048491
2316	30-31	-0.24048905302534962
2316	32-33	-0.04833576638932158
2316	34-35	-0.08025232257192982
2316	36-37	-0.3567541886022667
2316	38-39	-0.303227223600409
2316	40-41	-0.2356086095446841
2316	42-43	-0.2897528588097842
2316	44-45	-0.16765085274541036
2316	46-47	-0.28405587931296594
2316	48-49	-0.2613615009570651
2316	50-51	-0.34062914908514585
2316	52-53	-0.32372561980263015
2316	54-55	-0.11719573071170686
2316	56-57	-0.815881813682509
2316	58-59	-0.7191407252357394
2316	60-61	-0.3632203886369183
2316	62-63	-0.08925149952577982
2316	64-65	-0.03344132339756101
2316	66-67	0.0517426926654565
2316	68-69	-0.0285651485284788
2316	70-71	-0.0727315227698142
2316	72-73	0.014752626784826361
2316	74-75	-0.010582814588808276
2316	76-77	0.00830525661356063
2316	78-79	-0.01271459282224896
2316	80-81	-0.04225765983935048
2316	82-83	-0.25271270398015844
2316	84-85	-0.12367391123542149
2316	86-87	-0.17606328250404601
2316	88-89	-0.17735975587373076
2316	90-91	-0.08141549967236728
2316	92-93	-0.10984405231215533
2316	94-95	-0.10433970869942755
2316	96-97	-0.23553952004065337
2316	98-99	-0.09116346454391788
2316	100	-0.3622869979418972
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	7.0
13	46.0
14	191.0
15	522.0
16	1287.0
17	2743.0
18	4854.0
19	7796.0
20	11852.0
21	17880.0
22	24433.0
23	26706.0
24	27723.0
25	30202.0
26	36056.0
27	45187.0
28	56203.0
29	70429.0
30	86099.0
31	108365.0
32	136973.0
33	180312.0
34	262841.0
35	400843.0
36	725556.0
37	1479539.0
38	1668318.0
39	295402.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.83954695602841	20.000059774828223	13.486273677644547	23.674119591498823
2	15.912058650923647	20.707665087109234	38.87244124834672	24.5078350136204
3	18.8558682166034	25.498346611130927	30.29117723109394	25.354607941171732
4	13.215464135150452	17.37529792118676	38.795299129951886	30.6139388137109
5	13.937001073381488	37.73788223777003	34.16811357977369	14.157003109074795
6	30.279848027042327	37.25989702164396	18.29402156547282	14.166233385840895
7	27.597637158002797	32.430444364250455	21.931265963373146	18.040652514373605
8	26.722166533937408	34.21776684800298	19.980617907553867	19.079448710505744
9	26.057114767355046	16.1339792783257	20.639529689258975	37.169376265060286
10-11	25.120452289401523	25.84512367386862	28.0012518436709	21.03317219305896
12-13	26.005939510885966	23.309825506612427	27.9515344320392	22.73270055046241
14-15	23.497381388165582	24.985170892976765	25.282690254027067	26.234757464830576
16-17	23.00641066460467	27.873318141044784	25.989814795917965	23.13045639843258
18-19	22.60365481449183	27.268326211143762	27.796440064337517	22.33157891002689
20-21	23.699351487774933	26.363061211744466	27.53864126094762	22.398946039532976
22-23	23.675721611043986	26.08045234605377	27.235299795580982	23.00852624732126
24-25	23.371237814414727	26.307445329536343	27.470585370570728	22.8507314854782
26-27	23.293822595757618	26.510965344286312	27.032541066007713	23.16267099394835
28-29	22.974279631351777	26.523063640243517	27.416538362078935	23.086118366325774
30-31	23.53420208992455	26.247677353083414	27.332766137255437	22.8853544197366
32-33	23.46626032904727	26.463341923680645	26.960506303121345	23.10989144415074
34-35	23.564721338117355	26.53876514048748	27.20591062976561	22.69060289162955
36-37	23.53732993858762	26.243796915154483	26.987737847700384	23.23113529855751
38-39	23.52006518960446	26.271219625350746	27.251840662856758	22.956874522188038
40-41	23.528942317202254	25.877413487717355	27.49031968071262	23.103324514367767
42-43	23.363033933912128	25.924210341534277	27.28540002871398	23.42735569583962
44-45	23.414554801059932	26.25726529535403	27.148274565711482	23.179905337874555
46-47	23.42546108549817	26.198718932483445	26.722076112831488	23.653743869186897
48-49	23.75625158175717	26.063004328134383	26.92012508463414	23.260619005474314
50-51	23.560004610928388	26.22770348086552	26.753519249049347	23.45877265915674
52-53	23.360015500896363	26.329887735879304	26.69632591265294	23.613770850571395
54-55	23.326175644669373	26.278349939322865	26.72218561719779	23.67328879880997
56-57	23.702303291822094	26.177377846771936	26.495489476993754	23.624829384412216
58-59	23.16034737512314	26.326260278029384	26.963361676882837	23.55003066996464
60-61	23.333437274344355	26.550408645836654	26.62363450416459	23.4925195756544
62-63	23.399411527488386	26.22188805217911	26.52090678582803	23.857793634504475
64-65	23.01457194580726	26.18798444248319	26.75563374878205	24.0418098629275
66-67	23.307086476233522	26.13661416857288	26.634573062657417	23.921726292536178
68-69	23.055924585411855	26.147895020317396	26.801541373499976	23.994639020770773
70-71	23.11213409945342	25.911498992568845	26.74410670510406	24.232260202873675
72-73	23.173017132545215	26.11844881149225	26.69058768572637	24.017946370236164
74-75	23.21718409451273	26.28323457744178	26.61207535937506	23.887505968670425
76-77	23.026562551187784	26.277458769909472	26.432079821322112	24.263898857580628
78-79	22.951384138237064	26.16212306296764	26.56330338327773	24.32318941551756
80-81	22.84349696112475	26.507218708273932	26.6018342915183	24.047450039083014
82-83	23.253007795300082	26.055547677268255	26.922300398773697	23.769144128657967
84-85	23.28004042537742	26.114748264170977	26.533901407171367	24.071309903280238
86-87	22.643806884300602	26.40321506201897	26.801944418774443	24.15103363490599
88-89	22.931295431196233	25.86385568984469	26.76586861368342	24.43898026527566
90-91	22.933988628107222	25.920102446012415	26.74124717684419	24.404661749036176
92-93	22.994353281405175	26.02525353123564	26.690910435051173	24.28948275230801
94-95	22.442125843033224	25.92778874887059	27.010532687068086	24.619552721028104
96-97	22.56279869992339	25.88570916032661	26.894034086280644	24.657458053469355
98-99	22.345512122189703	26.015388371068195	26.826781101774856	24.81231840496725
100	22.62185663019019	25.706885391219192	26.59964665146643	25.071611327124188
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	62.5
2	106.0
3	158.0
4	129.0
5	121.5
6	260.0
7	541.0
8	907.5
9	1137.5
10	1084.0
11	975.5
12	1051.5
13	1283.5
14	1509.0
15	1717.0
16	2024.0
17	2356.5
18	2657.5
19	2970.5
20	3358.5
21	3838.0
22	4328.0
23	4939.5
24	5922.0
25	7227.0
26	8953.0
27	11324.5
28	14330.0
29	18700.0
30	25482.0
31	33933.0
32	44907.5
33	58806.5
34	73911.5
35	91297.5
36	115281.0
37	143603.5
38	162963.5
39	173976.0
40	178953.0
41	180456.0
42	187809.0
43	201285.5
44	216468.0
45	236430.0
46	254198.0
47	265979.0
48	274841.5
49	275303.5
50	267330.0
51	259430.0
52	251095.0
53	241325.0
54	230888.5
55	207981.0
56	182442.5
57	154422.5
58	128766.0
59	113950.5
60	93964.0
61	72199.5
62	56127.5
63	42932.0
64	30541.5
65	20228.0
66	14218.5
67	10295.5
68	7689.5
69	6025.5
70	4576.5
71	3368.0
72	2597.5
73	1968.0
74	1429.5
75	1007.0
76	656.5
77	389.5
78	249.5
79	150.0
80	85.0
81	51.5
82	36.5
83	22.0
84	15.5
85	11.0
86	7.5
87	6.5
88	4.0
89	2.5
90	2.0
91	2.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3568610661090774
2	5.956151513282919E-4
3	0.001891954010101633
4	0.0013839293222039724
5	0.004467113634962189
6	0.0
7	7.007237074450492E-5
8	3.8539803909477707E-4
9	0.0027678586444079448
10-11	6.131332440144181E-5
12-13	2.6277139029189344E-5
14-15	2.802894829780197E-4
16-17	9.634950977369427E-5
18-19	8.846636806493747E-4
20-21	1.8393997320432544E-4
22-23	1.9269901954738853E-4
24-25	0.0
26-27	0.0018306406857001912
28-29	0.010721072723909254
30-31	0.003284642378648668
32-33	0.014277245539192877
34-35	0.011097711716660966
36-37	0.012963388587733412
38-39	0.012709376243784582
40-41	0.013988197009871795
42-43	0.006350308598720759
44-45	0.001436483600262351
46-47	0.0058160067717939095
48-49	0.012761930521842959
50-51	0.0036087270933420033
52-53	0.005728416308363278
54-55	0.005307982083896248
56-57	1.8393997320432544E-4
58-59	5.343018269268501E-4
60-61	3.153256683502722E-4
62-63	8.058322635618066E-4
64-65	3.153256683502722E-4
66-67	0.0014277245539192878
68-69	0.001725532129583434
70-71	9.372179587077533E-4
72-73	0.0027065453200065025
74-75	0.007961973125844372
76-77	0.004116751781239664
78-79	0.0018043635466710017
80-81	3.8539803909477707E-4
82-83	1.4890378783207297E-4
84-85	0.0015503512027221715
86-87	0.004405800310560747
88-89	0.0016729778515250553
90-91	2.0145806589045166E-4
92-93	0.001979544473532264
94-95	0.003529895676254435
96-97	0.0018393997320432542
98-99	0.001970785427189201
100	0.002435014883371546
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5708384.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.86971735906127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70256002711992	45.47123714849097
2	14.816991085562492	18.038121189798385
3	4.83780749056604	8.83427923764913
4	2.122177972925905	5.167054935905016
5	1.0691441869896554	3.2539252239071836
6	0.6284636620287698	2.2952643286879093
7	0.41914173940718286	1.785912744777037
8	0.28201764343332897	1.3733067396844203
9	0.19214319451263012	1.0526131748205836
>10	0.8803829112715553	8.979759416944205
>50	0.033773821402070046	1.4004960867378073
>100	0.014463688432965666	1.5633824930156643
>500	6.90797942710808E-4	0.2573587968174293
>1k	1.7269886066251114E-4	0.19798547217793508
>5k	6.907954426374293E-5	0.32930301058629735
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8308	0.14554031403633672	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7355	0.12884557170645844	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.759046343063116E-5	0.0
3	0.0	0.0	0.0	1.7518092686126231E-4	0.0
4	0.0	0.0	0.0	3.328437610363984E-4	0.0
5	0.0	0.0	0.0	4.029161317809033E-4	0.0
6	0.0	0.0	0.0	4.029161317809033E-4	1.751809268612623E-5
7	0.0	0.0	0.0	4.7298850252540825E-4	1.751809268612623E-5
8	0.0	0.0	0.0	5.605789659560394E-4	1.751809268612623E-5
9	0.0	0.0	0.0	0.0011386760245982051	3.503618537225246E-5
10-11	0.0	0.0	0.0	0.0016467007124958658	3.503618537225246E-5
12-13	0.0	0.0	0.0	0.0018656768710724437	3.503618537225246E-5
14-15	0.0	0.0	0.0	0.0021722434930796525	9.634950977369428E-5
16-17	0.0	0.0	0.0	0.0024612920224007353	1.3138569514594675E-4
18-19	0.0	0.0	0.0	0.0028729672005247016	1.4014474148900985E-4
20-21	0.0	0.0	0.0	0.0033196785640209206	1.4014474148900985E-4
22-23	0.0	0.0	0.0	0.00404667941049516	1.664218805181992E-4
24-25	0.0	0.0	0.0	0.004791198349655524	2.27735204919641E-4
26-27	0.0	0.0	0.0	0.0059824286523121085	2.540123439488303E-4
28-29	0.0	0.0	0.0	0.008469997813742032	2.6277139029189344E-4
30-31	0.0	0.0	0.0	0.014907896875893422	2.7153043663495657E-4
32-33	0.0	0.0	0.0	0.027801213092882332	3.153256683502722E-4
34-35	0.0	0.0	0.0	0.052212675250999235	3.5036185372252463E-4
36-37	0.0	0.0	0.0	0.09222399894611155	3.678799464086509E-4
38-39	0.0	0.0	0.0	0.14809795556851116	3.678799464086509E-4
40-41	0.0	0.0	0.0	0.21125067970199624	4.029161317809033E-4
42-43	0.0	0.0	0.0	0.2805785315073408	4.029161317809033E-4
44-45	0.0	0.0	0.0	0.36862446534781124	4.029161317809033E-4
46-47	0.0	0.0	0.0	0.4778760503848375	4.467113634962189E-4
48-49	0.0	0.0	0.0	0.5970516349285542	5.080246878976607E-4
50-51	0.0	0.0	0.0	0.72511765151048	5.255427805837869E-4
52-53	0.0	0.0	0.0	0.8505384360968008	5.956151513282918E-4
54-55	0.0	0.0	0.0	0.9896320920246431	6.656875220727968E-4
56-57	0.0	0.0	0.0	1.1571050581040097	6.656875220727968E-4
58-59	0.0	0.0	0.0	1.3508288860735367	7.007237074450493E-4
60-61	0.0	0.0	0.0	1.5514285654223683	7.357598928173018E-4
62-63	0.0	0.0	0.0	1.75763403443076	7.532779855034279E-4
64-65	0.0	0.0	0.0	1.972335778391923	7.532779855034279E-4
66-67	0.0	0.0	0.0	2.214190566016582	7.532779855034279E-4
68-69	0.0	0.0	0.0	2.4843195552366484	7.532779855034279E-4
70-71	0.0	0.0	0.0	2.7839227354011227	8.321094025909961E-4
72-73	0.0	0.0	0.0	3.088649957676288	9.985312831091951E-4
74-75	0.0	0.0	0.0	3.3938326503612934	9.985312831091951E-4
76-77	0.0	0.0	0.0	3.727622388402742	9.985312831091951E-4
78-79	0.0	0.0	0.0	4.0936716941256925	9.985312831091951E-4
80-81	0.0	0.0	0.0	4.48664630830722	0.0010335674684814476
82-83	0.0	0.0	0.0	4.900511248016953	0.001129916978255142
84-85	0.0	0.0	0.0	5.311494461479816	0.0011386760245982051
86-87	0.0	0.0	0.0	5.735598726364589	0.0011386760245982051
88	0.0	0.0	0.0	6.06933591012798	0.0011386760245982051
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8030	0.0	45.760952	1
GTATCAA	14020	0.0	39.095905	1
CAACGCA	15670	0.0	33.1707	5
ATCAACG	15955	0.0	32.49067	3
TATCAAC	17220	0.0	31.276909	2
AACGCAG	17450	0.0	30.324152	6
TCAACGC	17735	0.0	29.494955	4
GTACTAG	2885	0.0	28.776634	1
ACGCAGA	19440	0.0	26.760912	7
CGCAGAG	19840	0.0	26.126629	8
GTACATG	22040	0.0	22.793509	1
GCAGAGT	22305	0.0	22.649153	9
TACATGG	22125	0.0	21.751492	2
GTATAAG	3595	0.0	21.38759	1
GTGGTAT	3650	0.0	21.19455	1
ACATGGG	21910	0.0	21.000048	3
TAGGCAT	4260	0.0	20.961027	5
GAGTACT	9865	0.0	19.721823	12-13
GCATCGT	3475	0.0	19.60933	8
CAGAGTA	21895	0.0	19.080477	10-11
>>END_MODULE