FastQCFastQC Report
Thu 26 May 2016
ERR580059_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580059_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7197655
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159640.2217944594454722No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128210.17812745956842888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78600.10920223322734973No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA107000.041.1389241
GTATCAA175050.037.934421
GTACTAG57200.037.4090161
CAACGCA190300.032.7161185
ATCAACG198950.031.3205783
GCATCGT60800.031.0761138
TAGTACT79050.030.3403174
TATCAAC220400.029.8517672
GTACATG271300.029.8326571
GGGCACC70900.029.62983994
TAGGCAT74450.029.1803055
CTGTGCG84300.029.1050479
AACGCAG222650.028.8159076
TACATGG277900.028.5828172
ACATGGG272650.027.8563923
CTAGGCA80950.027.7110754
CTAACGC28800.027.4330863
TCTAACG28700.027.365192
TAACGCC28550.027.3434814
TCAACGC234500.026.8527984
Produced by FastQC (version 0.11.5)