FastQCFastQC Report
Thu 26 May 2016
ERR580059_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580059_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7197655
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142960.1986202450659277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117840.16371998935764495No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81410.11310628253229699No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106400.043.4734271
GTATCAA177050.038.6842351
GTACTAG57600.035.9903871
CAACGCA202150.032.1318175
ATCAACG211050.030.8665373
TATCAAC229350.029.818542
GGGCACC69800.029.56490394
TAGGCAT75400.028.8609525
AACGCAG235750.028.3483496
GTACATG269950.028.0183871
CTAGGCA80700.027.8394994
TAGTACT82750.027.6042064
CTGTGCG83600.027.4904869
TACATGG272600.027.3808382
TCAACGC245500.026.8604454
ACATGGG272150.026.2509673
GTGGTAT35500.026.0860461
CTAGTAC89300.025.8428333
GCATCGT74600.025.7040318
ACGCAGA253950.025.6504447
Produced by FastQC (version 0.11.5)