FastQCFastQC Report
Thu 26 May 2016
ERR580058_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580058_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4421620
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74070.16751778759821062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63080.14266264400830464No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68450.049.3896561
GTATCAA115700.039.217011
CAACGCA134000.032.1725965
ATCAACG136850.031.6085033
TATCAAC142900.031.0942542
AACGCAG147400.029.7782346
TCAACGC148700.029.1834664
ACGCAGA163300.026.2454097
CGCAGAG169200.025.2741098
GTACTAG23050.024.8868241
GTACATG174750.022.7632221
GCAGAGT185800.022.4876949
GTGGTAT31450.022.1269511
GTATAAG24700.021.8917981
TACATGG179600.021.5987262
TGATCGC14100.020.9995679
GCATCGT23350.020.7293248
GTGATCG14500.020.4177278
GAGTACT79800.020.32522212-13
ACATGGG186550.020.2386343
Produced by FastQC (version 0.11.5)