FastQCFastQC Report
Thu 26 May 2016
ERR580058_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580058_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4421620
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64810.14657523713028256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58200.13162596514399702No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67400.047.58031
GTATCAA113600.036.7403341
CAACGCA128250.031.6620355
ATCAACG131550.030.9049023
TATCAAC139650.029.7174822
AACGCAG142550.029.1432516
TCAACGC141850.028.6929974
ACGCAGA158800.025.599037
GTACTAG20850.025.3332021
CGCAGAG165000.024.4947178
GTACATG172400.022.513381
TACATGG174500.021.7086052
GCAGAGT185750.021.555789
ACATGGG176950.020.8241653
CTAGTAC31800.020.6920263
TAGTACT31150.020.5195674
GTGGTAT31600.020.4461541
CTGTGCG29700.020.4120339
GAGTACT79350.019.98893512-13
GGGCTAT28500.019.9523246
Produced by FastQC (version 0.11.5)