FastQCFastQC Report
Thu 26 May 2016
ERR580057_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580057_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903225
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39330.135470037630566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33550.11556114321142866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39700.047.6163441
GTATCAA66000.036.2655561
CAACGCA73850.030.6224675
ATCAACG75350.029.8277343
TATCAAC82050.028.3104272
AACGCAG82400.028.116636
TCAACGC82850.027.4113374
ACGCAGA94850.023.9788937
GTACATG107550.023.9164891
CGCAGAG97900.023.2794488
TACATGG108500.022.9257722
GTACTAG13250.021.6488321
GTGGTAT17000.021.022541
ACATGGG113050.020.9621373
CATGGGG88050.020.3456334
GTGTAGC16800.020.1531871
GCAGAGT109700.019.9652739
GCATCGT14000.019.1320048
GAGTACT44350.018.55069412-13
GAGTACA86150.018.2856351
Produced by FastQC (version 0.11.5)