FastQCFastQC Report
Thu 26 May 2016
ERR580057_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580057_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903225
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35940.12379336772037992No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31430.10825891896081082No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38650.048.6914331
GTATCAA66650.037.789421
CAACGCA76200.031.1468685
ATCAACG76850.031.0084083
TATCAAC82850.029.952072
TCAACGC82300.029.0672134
AACGCAG85950.028.4333466
ACGCAGA96300.024.791797
GTACATG114800.023.7473471
GTGGTAT17950.023.6486991
CGCAGAG101400.023.3131268
GTACTAG12100.022.9983041
TACATGG116750.022.9860672
ACATGGG118550.022.043913
GCAGAGT115600.020.2864349
TGGTATC19000.019.7889082
CATGGGG92100.019.5955314
GAGTACA89150.018.8346611
CAGAGTA108900.018.38293310-11
AGAGTAC100650.018.34897610-11
Produced by FastQC (version 0.11.5)