FastQCFastQC Report
Thu 26 May 2016
ERR580056_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580056_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6951658
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107210.15422220137987225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82050.1180293967280899No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83050.050.0509831
GTATCAA160450.044.8971751
CAACGCA194000.036.350325
ATCAACG197300.035.817683
TATCAAC202950.035.3098532
AACGCAG214900.033.4345866
TCAACGC215750.032.8193784
ACGCAGA235800.029.6336947
CGCAGAG243150.028.5247178
GTACATG269100.025.0398921
GCAGAGT276050.024.4993299
TACATGG275100.023.998082
ACATGGG279300.022.9956323
TATTCGC5300.021.28229
CATGGGG216200.020.9424174
AGAGTAC240700.020.62158810-11
GAGTACT107550.020.56678212-13
CGTTAAC10250.020.1850011
CAGAGTA263150.019.5767910-11
GTGGTAT39950.018.714591
Produced by FastQC (version 0.11.5)