FastQCFastQC Report
Thu 26 May 2016
ERR580056_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580056_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6951658
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94900.13651419560628558No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76510.11006007487710126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87500.051.6479571
GTATCAA158700.042.0308461
CAACGCA191250.033.4204185
ATCAACG195300.032.7766763
TATCAAC202200.032.448242
AACGCAG212250.030.8205876
TCAACGC213500.030.1142444
ACGCAGA238300.026.6034097
CGCAGAG246600.025.6321418
GTACATG266250.023.635341
GTGGTAT36700.023.5223481
TACATGG277550.022.4029662
GCAGAGT277500.022.1510439
ACATGGG279150.021.5675853
CGTTAAC11300.020.4556521
GTATAAG35450.019.9604641
GTACTAG26250.019.7678321
TGATCGC21050.019.199769
GTGATCG21300.019.1953188
GAGTACT109800.019.17399412-13
Produced by FastQC (version 0.11.5)