FastQCFastQC Report
Thu 26 May 2016
ERR580055_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580055_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147480
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52740.16756262152579207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45220.14367049194911483No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50700.052.5014081
GTATCAA87400.039.603071
CAACGCA101000.032.9524575
ATCAACG102400.032.5024533
TATCAAC107500.031.6244642
AACGCAG108500.031.1348786
TCAACGC109650.030.6535114
ACGCAGA123000.026.7013917
GTACTAG14100.026.349381
CGCAGAG126100.025.821418
GTACATG138050.023.6761381
TACATGG139500.023.3251912
GCAGAGT141700.022.153259
ACATGGG143550.021.9736463
GTGGTAT21150.021.5686911
GAGTACT55850.021.20555512-13
CAGAGTA134750.020.3504510-11
GCATCGT15050.020.2926458
CATGGGG116000.020.1004834
TAGTACT18300.020.0367074
Produced by FastQC (version 0.11.5)