FastQCFastQC Report
Thu 26 May 2016
ERR580055_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580055_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3147480
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48350.15361495545642864No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.12876968241259673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51600.049.207471
GTATCAA86200.038.456421
ATCAACG103200.031.1988873
CAACGCA103450.031.1235
TATCAAC106900.030.7883632
TCAACGC111100.028.9381184
AACGCAG112700.028.8563526
GTGGTAT19150.025.5359211
ACGCAGA126050.025.3527647
GTACATG140300.024.4989071
CGCAGAG130300.024.3451088
TACATGG141250.023.8336662
ACATGGG144900.022.5122743
CATGGGG117900.021.6478254
GCAGAGT146450.021.2750029
GAGTACT57050.020.63532612-13
ATGGGGG84650.020.5445255
ACGTTAA5650.019.962518
GACGTTA5900.019.9134857
TGGTATC23900.019.8696292
Produced by FastQC (version 0.11.5)