FastQCFastQC Report
Thu 26 May 2016
ERR580054_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580054_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12424669
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT321430.2587030688704866No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254870.20513222525284175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142500.11469118412731961No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA216600.046.455461
GTATCAA390900.040.9935341
CAACGCA434250.035.0877845
ATCAACG445400.034.2971653
TATCAAC483200.032.958672
AACGCAG498900.031.5260856
GTACTAG103800.031.074791
GTGGTAT67650.030.3040121
TCAACGC531550.029.1009374
ACGCAGA525850.028.8638657
CGCAGAG535900.028.3043468
GGGCACC122150.027.96689894
TAGGCAT127700.027.5008685
CTGTGCG140700.026.6239519
GCAGAGT577800.025.989729
TAGTACT145850.025.6280694
CTAGGCA146700.025.0308824
GCATCGT116650.025.015568
GTACATG583350.024.1164741
ACTGTGC169200.024.1058648
Produced by FastQC (version 0.11.5)