FastQCFastQC Report
Thu 26 May 2016
ERR580054_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580054_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12424669
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT288640.2323120237649792No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243970.19635935573012048No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139010.11188225617921893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222200.046.8232151
GTATCAA395650.040.350351
CAACGCA452300.033.7082375
ATCAACG461000.033.0530323
GTGGTAT70400.032.4244421
TATCAAC500450.031.7612922
GTACTAG104200.031.0496121
AACGCAG517250.030.4278986
TCAACGC545700.028.3180334
TAGGCAT134150.027.9572185
ACGCAGA550500.027.6852637
GGGCACC115350.027.05350394
CGCAGAG562250.027.0147488
CTAGGCA150200.025.7835394
GCAGAGT605250.024.7772319
CTGTGCG140250.024.3608449
CGTTAAC27600.023.9231741
TAGTACT146300.023.8366284
GTACATG573350.023.6739521
GCATCGT130850.023.2015938
Produced by FastQC (version 0.11.5)