##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580054_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12424669
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.40862480924039	34.0	31.0	34.0	31.0	34.0
2	32.628081923148216	34.0	31.0	34.0	31.0	34.0
3	32.704894593167836	34.0	31.0	34.0	31.0	34.0
4	36.21479292526827	37.0	35.0	37.0	35.0	37.0
5	36.13792125971324	37.0	35.0	37.0	35.0	37.0
6	36.170657825975084	37.0	37.0	37.0	35.0	37.0
7	36.13345981289321	37.0	36.0	37.0	35.0	37.0
8	36.12781934070034	37.0	36.0	37.0	35.0	37.0
9	37.893408428023314	39.0	38.0	39.0	35.0	39.0
10-11	37.835459761543746	39.0	38.0	39.0	35.0	39.0
12-13	37.78568728873179	39.0	38.0	39.0	35.0	39.0
14-15	39.13362512916843	40.5	39.0	41.0	36.0	41.0
16-17	39.12021692489353	40.0	38.5	41.0	36.0	41.0
18-19	39.12274540271455	40.0	38.5	41.0	35.5	41.0
20-21	39.10744318420072	40.0	39.0	41.0	35.0	41.0
22-23	39.0234924970637	40.0	38.5	41.0	35.0	41.0
24-25	38.92811281330714	40.0	38.0	41.0	35.0	41.0
26-27	38.769967554065225	40.0	38.0	41.0	35.0	41.0
28-29	38.664308642749354	40.0	38.0	41.0	35.0	41.0
30-31	38.54294058054987	40.0	38.0	41.0	34.0	41.0
32-33	38.38906014317162	40.0	38.0	41.0	34.0	41.0
34-35	38.351239336838674	40.0	38.0	41.0	34.0	41.0
36-37	38.392674042262215	40.0	38.0	41.0	34.0	41.0
38-39	38.283444130382875	40.0	38.0	41.0	34.0	41.0
40-41	38.1370556833345	40.0	37.5	41.0	33.5	41.0
42-43	38.08813244843786	40.0	37.0	41.0	33.0	41.0
44-45	37.952353177376395	40.0	37.0	41.0	33.0	41.0
46-47	37.7104177181702	40.0	37.0	41.0	33.0	41.0
48-49	37.44846277192576	40.0	36.5	41.0	32.5	41.0
50-51	37.24514254665456	40.0	36.0	41.0	32.0	41.0
52-53	37.037028511584495	39.0	35.5	41.0	31.5	41.0
54-55	36.81172154364836	39.0	35.0	41.0	31.0	41.0
56-57	36.56302276543544	39.0	35.0	40.5	31.0	41.0
58-59	36.29356520483564	38.0	35.0	40.0	31.0	41.0
60-61	36.02467087855621	37.5	35.0	40.0	30.5	41.0
62-63	35.72608916181187	37.0	35.0	40.0	30.0	41.0
64-65	35.433649017128744	36.5	34.5	40.0	30.0	41.0
66-67	35.12113863958871	36.0	34.0	39.0	29.0	41.0
68-69	34.80078020589522	35.5	34.0	39.0	29.0	41.0
70-71	34.525250974492764	35.0	34.0	38.5	29.0	40.0
72-73	34.21512593212745	35.0	34.0	37.5	29.0	40.0
74-75	33.89023083834266	35.0	34.0	37.0	28.0	39.5
76-77	32.69628060111702	34.5	32.0	36.0	26.5	38.5
78-79	33.390535836407395	35.0	33.0	36.0	28.0	39.0
80-81	33.36812288520523	35.0	33.0	36.0	29.0	37.5
82-83	33.17435563072143	35.0	33.0	35.5	28.0	37.0
84-85	32.967750770664395	35.0	33.0	35.0	27.5	37.0
86-87	32.79714554166392	35.0	33.0	35.0	27.0	36.5
88-89	32.63639389508083	35.0	33.0	35.0	27.0	36.0
90-91	32.464869848846675	35.0	33.0	35.0	27.0	36.0
92-93	32.32958061096034	35.0	33.0	35.0	26.5	35.5
94-95	32.23073942653925	35.0	33.0	35.0	26.0	35.0
96-97	32.1232515731405	35.0	33.0	35.0	25.5	35.0
98-99	32.039435778933026	35.0	32.0	35.0	25.0	35.0
100	30.68748624208822	34.0	30.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.1479478031262147
1101	2	0.060344932031959786
1101	3	0.027559118660441584
1101	4	0.012661902250968637
1101	5	-4.784950038967395E-4
1101	6	0.026418736068592352
1101	7	0.012643840791234595
1101	8	0.03455223544201402
1101	9	0.016353149115552412
1101	10-11	-0.018149573695509957
1101	12-13	-0.02912269167040904
1101	14-15	0.02975618175859296
1101	16-17	-0.0318984971709213
1101	18-19	-0.03409480518274677
1101	20-21	-0.038583571855383525
1101	22-23	-0.06715492615206387
1101	24-25	-0.08942081736877583
1101	26-27	-0.11695746258796191
1101	28-29	-0.12400613911569991
1101	30-31	-0.11277111805134155
1101	32-33	-0.08203902107224081
1101	34-35	-0.13376117764211415
1101	36-37	-0.0942239258803923
1101	38-39	-0.010628123190876693
1101	40-41	-0.013580170443276529
1101	42-43	-0.10517724691896291
1101	44-45	-0.154860551685978
1101	46-47	-0.03629030442824899
1101	48-49	-0.07363318569368005
1101	50-51	-0.10470638820407174
1101	52-53	-0.1264743270527191
1101	54-55	-0.08838486814492796
1101	56-57	-0.1433240505148845
1101	58-59	-0.1044601019166791
1101	60-61	-0.20972214246370413
1101	62-63	-0.15519366705267856
1101	64-65	-0.1204274721661065
1101	66-67	-0.23281411322929557
1101	68-69	-0.19895144905880358
1101	70-71	-0.21785282692516006
1101	72-73	-0.1815646753466993
1101	74-75	-0.17714046929384608
1101	76-77	-0.16345665782503005
1101	78-79	-0.1365257618257374
1101	80-81	-0.10924133007913639
1101	82-83	-0.11308646958347879
1101	84-85	-0.16730592363962415
1101	86-87	-0.15543540704197056
1101	88-89	-0.13779960473797104
1101	90-91	-0.12649909467420883
1101	92-93	-0.19274801206275782
1101	94-95	-0.18318016599970122
1101	96-97	-0.17905701352295722
1101	98-99	-0.20223133322824083
1101	100	-0.11494025945237496
1102	1	0.09773369026514445
1102	2	0.05836450389296033
1102	3	0.01855789282065956
1102	4	0.041072073636414075
1102	5	0.008221549054006516
1102	6	0.028301242483095734
1102	7	0.04457925049209166
1102	8	0.006650948363038367
1102	9	0.004277917164245082
1102	10-11	-0.013888719668742056
1102	12-13	-0.009882825427034447
1102	14-15	0.03524660573935989
1102	16-17	0.012804888197159414
1102	18-19	0.01800983677623691
1102	20-21	0.0017707662022914405
1102	22-23	-0.0258257664552346
1102	24-25	-0.02177231379697986
1102	26-27	-0.05463324659486091
1102	28-29	-0.09079287965195704
1102	30-31	-0.05398598637552254
1102	32-33	-0.07908023169117229
1102	34-35	-0.04648076478182617
1102	36-37	0.0182034475128674
1102	38-39	-0.0533177056821188
1102	40-41	0.0037017299249697544
1102	42-43	0.01469090915178839
1102	44-45	0.026029543293532242
1102	46-47	0.04098254192940942
1102	48-49	-0.016935804291918544
1102	50-51	-0.018838233170512808
1102	52-53	-0.04551228034971899
1102	54-55	-0.06122652591098188
1102	56-57	-0.10424352900972167
1102	58-59	-0.09232952657112747
1102	60-61	-0.0677901918265249
1102	62-63	-0.07288157580847354
1102	64-65	-0.11461794032973671
1102	66-67	-0.12915491109678356
1102	68-69	-0.08788400172839061
1102	70-71	-0.07284142487382184
1102	72-73	-0.07539032447910188
1102	74-75	-0.1435129482007369
1102	76-77	-0.12247849365105168
1102	78-79	-0.02557474337575627
1102	80-81	-0.04478149325989733
1102	82-83	-0.02809890402421189
1102	84-85	-0.05453262513439938
1102	86-87	-0.09729255865283193
1102	88-89	-0.05797167430181105
1102	90-91	-0.08574172299501726
1102	92-93	-0.06475510518957606
1102	94-95	-0.08986880711277223
1102	96-97	-0.17125168611002906
1102	98-99	-0.18064631291276356
1102	100	-0.1354322422642653
1103	1	0.12599052622188367
1103	2	0.07750485171957422
1103	3	0.04527713332088723
1103	4	0.04125092598422242
1103	5	0.017059363496350954
1103	6	0.04771682101255692
1103	7	0.019643387910072363
1103	8	0.0417926696001274
1103	9	0.009072267389910849
1103	10-11	0.006827663973737685
1103	12-13	0.011847028578735319
1103	14-15	0.051543268708179824
1103	16-17	0.03635548848883019
1103	18-19	0.036464469380263154
1103	20-21	0.0174675142683256
1103	22-23	-0.025565029710058695
1103	24-25	-0.0253399303796229
1103	26-27	-0.02671034549538831
1103	28-29	-0.02534721252396821
1103	30-31	-0.058791636654319746
1103	32-33	-0.06289355191257329
1103	34-35	-0.057583820541310615
1103	36-37	-0.01672560949864277
1103	38-39	-6.79381318569483E-4
1103	40-41	-0.03639202205052072
1103	42-43	-0.10143544902615531
1103	44-45	0.005725709331166229
1103	46-47	-0.02052231994682785
1103	48-49	0.01784120646883025
1103	50-51	-0.011483263787695819
1103	52-53	-0.02914884491286074
1103	54-55	-0.015773250795206195
1103	56-57	-0.009839003817681657
1103	58-59	-0.02808283796121458
1103	60-61	-0.03009307266220418
1103	62-63	-0.06469428058056081
1103	64-65	-0.019440767698249317
1103	66-67	-0.03658492935374369
1103	68-69	-0.020452539435616757
1103	70-71	-0.01181932813067732
1103	72-73	-0.05933216073458425
1103	74-75	-0.14690101248807252
1103	76-77	-0.09957596906343014
1103	78-79	0.027791914184234656
1103	80-81	0.025216670166180677
1103	82-83	0.044084772916605175
1103	84-85	0.03182464477402647
1103	86-87	0.03592023657642329
1103	88-89	0.06495025964049006
1103	90-91	-0.008692950685563972
1103	92-93	-0.038998755964335885
1103	94-95	0.0671348911007641
1103	96-97	0.006539632743958634
1103	98-99	-0.006785798259031139
1103	100	0.06343625163318833
1104	1	0.09935275317599235
1104	2	0.07983826537110161
1104	3	0.06356447205986626
1104	4	0.040371433888239494
1104	5	-0.012362996932466785
1104	6	0.039850681882079186
1104	7	0.039869634065198056
1104	8	0.027230441704176656
1104	9	0.023983133813949564
1104	10-11	0.018818521827014933
1104	12-13	0.02484837305824783
1104	14-15	0.057126131578336015
1104	16-17	-0.010288139675068919
1104	18-19	-0.01433130080713596
1104	20-21	-0.02983838582263587
1104	22-23	-0.06263335799538083
1104	24-25	-0.050272576900816546
1104	26-27	-0.050562636408415074
1104	28-29	-0.04548841034297624
1104	30-31	-0.05097401808622237
1104	32-33	-0.12085451099031985
1104	34-35	-0.10398716910370354
1104	36-37	-0.06187161463638802
1104	38-39	-0.03682661948452193
1104	40-41	-0.01978675040365374
1104	42-43	0.003947397989655599
1104	44-45	-0.07257045132825368
1104	46-47	-0.07537075794105164
1104	48-49	-0.06600657929978127
1104	50-51	-0.07683137252963945
1104	52-53	-0.07750243439060256
1104	54-55	-0.16944700770106635
1104	56-57	-0.11134620223789682
1104	58-59	-0.08416554425011924
1104	60-61	-0.1307046734875641
1104	62-63	-0.05327501096336107
1104	64-65	-0.0447821265722439
1104	66-67	-0.04896156570617194
1104	68-69	-0.031719450405645944
1104	70-71	-0.049432763193415497
1104	72-73	-0.07514532752931302
1104	74-75	-0.14385583311032946
1104	76-77	-0.13260473684363916
1104	78-79	-0.09431762361462859
1104	80-81	-0.0837542810707319
1104	82-83	-0.019004123629109415
1104	84-85	-0.04998576399326993
1104	86-87	-0.030630604449278565
1104	88-89	-0.02734514283609002
1104	90-91	-0.00836963201096097
1104	92-93	-0.05935274729468176
1104	94-95	-0.11187688113984962
1104	96-97	-0.118455693364929
1104	98-99	-0.07801654249475831
1104	100	-0.11155991428484668
1105	1	0.08915926791986806
1105	2	0.03826654160982912
1105	3	-4.6426002659671894E-4
1105	4	-0.004582175430655866
1105	5	-0.01719245983181139
1105	6	0.010860114403257626
1105	7	-3.982133819206979E-4
1105	8	-0.038905331378963126
1105	9	-0.017277187489447954
1105	10-11	-0.02789105340183795
1105	12-13	-0.008615132628669642
1105	14-15	0.02451741727320922
1105	16-17	-0.03363774831996835
1105	18-19	-0.052318863960813644
1105	20-21	-0.07218726562906141
1105	22-23	-0.038999493427091636
1105	24-25	-0.0816296966507366
1105	26-27	-0.07149095993138133
1105	28-29	-0.05928740869213556
1105	30-31	-0.08859640545524883
1105	32-33	-0.07310950167632768
1105	34-35	-0.053281139316212034
1105	36-37	-0.013322970104034937
1105	38-39	0.006127461661868949
1105	40-41	-0.0010037688788884225
1105	42-43	-0.005196690876296373
1105	44-45	0.028962646669505432
1105	46-47	-0.02437380169279635
1105	48-49	-0.08448906987730709
1105	50-51	-0.04303793203899886
1105	52-53	-0.053423581319535174
1105	54-55	-0.062042169959198645
1105	56-57	-0.08429671807586914
1105	58-59	-0.06611380615996154
1105	60-61	-0.02839955843402464
1105	62-63	-0.018643573837145766
1105	64-65	-0.008773215792260203
1105	66-67	-0.019377314251336486
1105	68-69	0.003977535098862006
1105	70-71	-0.027827766090588568
1105	72-73	-0.037527762383710694
1105	74-75	-0.06151103251126955
1105	76-77	-0.07439013307832454
1105	78-79	-0.0038523187278656223
1105	80-81	0.01375095948363736
1105	82-83	0.03591873448178973
1105	84-85	0.0708232402541924
1105	86-87	0.05621747007966604
1105	88-89	0.0836718600837898
1105	90-91	0.1178067037210937
1105	92-93	0.06460348352387513
1105	94-95	0.024506176359537335
1105	96-97	0.016692367500020566
1105	98-99	0.012130015884693535
1105	100	0.15870814136668798
1106	1	0.09730398679661789
1106	2	0.06974614289345027
1106	3	0.015109021209916307
1106	4	0.015214415762045519
1106	5	-0.014359014614790055
1106	6	0.0040074962674481185
1106	7	0.008117020402181652
1106	8	-0.017643455571587197
1106	9	-0.01610694252321565
1106	10-11	-0.03070242525940614
1106	12-13	-0.0023785901396351505
1106	14-15	0.035293419670644255
1106	16-17	-0.05190783778623853
1106	18-19	-0.009066236109525505
1106	20-21	-0.06858883112561642
1106	22-23	-0.05044896097514595
1106	24-25	-0.03314890715331131
1106	26-27	-0.09256467681060343
1106	28-29	-0.04298544506097102
1106	30-31	-0.022697990772286403
1106	32-33	-0.08036232329565252
1106	34-35	-0.022466658751795876
1106	36-37	-0.03758211828540681
1106	38-39	-0.06289475798973854
1106	40-41	-0.08079110193726535
1106	42-43	-0.06979033432845227
1106	44-45	-0.05153627685269413
1106	46-47	-0.0796906404605906
1106	48-49	-0.02873988679621675
1106	50-51	-0.0425487674400884
1106	52-53	-0.035297697904397296
1106	54-55	-0.05404585704097542
1106	56-57	-0.10903715718360729
1106	58-59	-0.10483995227823328
1106	60-61	-0.0658196595034255
1106	62-63	-0.12891077637630133
1106	64-65	-0.07414375288374231
1106	66-67	-0.0231768212443626
1106	68-69	-0.03826982876387319
1106	70-71	-0.0683232024281395
1106	72-73	-0.07077504776817278
1106	74-75	-0.07856785949827838
1106	76-77	-0.05812367327971657
1106	78-79	-0.024385678290769874
1106	80-81	0.02006281723510739
1106	82-83	0.021909286456271104
1106	84-85	0.008590563206077206
1106	86-87	0.06373639044709734
1106	88-89	0.055531939008936604
1106	90-91	0.0752622202557589
1106	92-93	0.011316086162302952
1106	94-95	0.018241732947231526
1106	96-97	0.017299191043100848
1106	98-99	0.019996605509369658
1106	100	0.005150604115339519
1107	1	0.06393285291934347
1107	2	0.0317222936828756
1107	3	-0.016314785187404368
1107	4	-0.006639526273218621
1107	5	-0.005921051883703399
1107	6	0.005216388405102634
1107	7	-0.01662749741627323
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2316	12-13	-0.01132427971837302
2316	14-15	-0.04578698934074765
2316	16-17	-0.033624424716670376
2316	18-19	-0.059731268969144935
2316	20-21	-0.037517355132180796
2316	22-23	-0.05459074804921471
2316	24-25	-0.046346398604192984
2316	26-27	-0.019885893127707277
2316	28-29	-0.02697955676740804
2316	30-31	-0.37419800784521584
2316	32-33	-0.19955654448873617
2316	34-35	-0.2894443263941042
2316	36-37	-0.5334496485017084
2316	38-39	-0.4159715495295089
2316	40-41	-0.30296106330426653
2316	42-43	-0.4651521772367815
2316	44-45	-0.3218970216309103
2316	46-47	-0.4951605383191051
2316	48-49	-0.4711705779998425
2316	50-51	-0.4538751761959645
2316	52-53	-0.4793037013510073
2316	54-55	-0.29613049132949243
2316	56-57	-1.010404637116956
2316	58-59	-0.8693563784155103
2316	60-61	-0.5391906389010757
2316	62-63	-0.3380691417349695
2316	64-65	-0.26860482378818773
2316	66-67	-0.2144316826853796
2316	68-69	-0.3394559806570925
2316	70-71	-0.3298420492009697
2316	72-73	-0.17738945888261526
2316	74-75	-0.16403285492964415
2316	76-77	-0.21378117761663873
2316	78-79	-0.2924802305278291
2316	80-81	-0.24973069289372773
2316	82-83	-0.44417471208071646
2316	84-85	-0.27358277741871717
2316	86-87	-0.26401692705514535
2316	88-89	-0.27315025911871516
2316	90-91	-0.22107774454347862
2316	92-93	-0.2817585602365895
2316	94-95	-0.2318699897282812
2316	96-97	-0.3788551420987716
2316	98-99	-0.1860603567425727
2316	100	-0.4463708438952274
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	3.0
12	23.0
13	123.0
14	514.0
15	1660.0
16	4145.0
17	9299.0
18	17430.0
19	26692.0
20	41082.0
21	63423.0
22	81235.0
23	80212.0
24	73216.0
25	75210.0
26	87197.0
27	106964.0
28	132083.0
29	163287.0
30	201549.0
31	250664.0
32	317596.0
33	420274.0
34	640152.0
35	969946.0
36	1751145.0
37	3226420.0
38	3027585.0
39	655475.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.44419792634543	21.222006919397842	14.066946317114107	23.266848837142614
2	15.52117878364574	20.1441269275061	40.55391222126677	23.780782067581388
3	18.591748018618187	26.421801920574094	29.593711089701646	25.39273897110607
4	12.758982211118001	17.1261270673062	39.168990435190075	30.945900286385726
5	13.38990083382331	38.21951619011284	34.500098397509035	13.890484578554815
6	30.07283332859813	38.02971330664825	17.79400320443144	14.10345016032218
7	27.072696223044133	32.373299169023674	22.796499413827114	17.75750519410508
8	26.30337059479911	35.01409941155601	19.694249294466026	18.98828069917886
9	26.214237617288315	15.651974864092924	21.488191928429277	36.64559559018949
10-11	25.65697458582879	26.137748208452344	27.050424910938226	21.154852294780643
12-13	25.898218490987702	23.800749211437395	27.678910579751314	22.622121717823585
14-15	23.34367058624305	25.047059706026136	25.42013685384255	26.189132853888264
16-17	22.11742522287941	27.9249542200605	26.55324622267208	23.404374334388013
18-19	21.651745938484694	27.619499297761287	28.891915602577157	21.83683916117686
20-21	23.13337568999943	26.672421625143826	28.339325991368124	21.85487669348862
22-23	23.46351192697677	26.33044603513548	28.03843392276112	22.167608115126633
24-25	22.878086329704235	26.513829060556866	28.57771100381024	22.03037360592866
26-27	22.664497712388275	26.7939297450533	27.90184459709811	22.639727945460315
28-29	21.987589838840968	27.055475452854168	28.40900204555356	22.547932662751304
30-31	22.799231905191846	26.605961447564532	28.30479270474932	22.290013942494305
32-33	23.02203110972264	26.92162148825885	27.594240426153792	22.462106975864717
34-35	22.98317944959085	26.852345138490136	28.221149711161353	21.94332570075766
36-37	22.75642505260073	26.504126053549005	27.944377997724313	22.795070896125953
38-39	22.885705436989447	26.535874004047706	28.36418614600747	22.214234412955385
40-41	22.66451060387977	25.812546340632537	28.875924266959718	22.64701878852798
42-43	22.581186912057046	26.034549197704177	28.294234227599237	23.09002966263954
44-45	22.652050162044834	26.761771013039688	28.038519236216754	22.547659588698725
46-47	22.753450747800365	26.576952757529565	27.466368854479505	23.203227640190562
48-49	23.367419955293784	26.441196723411608	27.521099898982577	22.670283422312032
50-51	23.061466586334234	26.432829044883395	27.299061497332765	23.206642871449606
52-53	22.55593899480588	26.866875802909355	27.22021729225834	23.356967910026423
54-55	22.59619656730017	26.813037466073837	27.14920026014082	23.441565706485168
56-57	23.320336172410354	26.49403063019188	26.819005932286228	23.36662726511154
58-59	22.44260982244901	26.788870466654863	27.53666516427088	23.231854546625243
60-61	22.600187788469633	27.206667440399375	26.996403430672878	23.196741340458114
62-63	22.911343782414438	26.662187626702405	26.70323945659949	23.72322913428367
64-65	22.19258931356077	26.747422960451523	27.070491061098423	23.98949666488928
66-67	22.76984606739163	26.45302579891473	27.019093048888788	23.758035084804852
68-69	22.34051201185273	26.4574616333982	27.268590277997994	23.93343607675107
70-71	22.344766283801206	26.220377613493795	27.267308385104467	24.16754771760053
72-73	22.81603354974261	26.344578985468637	27.270027650656804	23.569359814131953
74-75	22.8066438228894	26.753511978212863	27.013535986434917	23.426308212462825
76-77	22.582171321655487	26.678687312912697	26.691770671858052	24.047370693573768
78-79	22.449839974942975	26.633506541390673	26.805509985725333	24.11114349794102
80-81	22.164107235149554	27.07377777870783	27.140532434782717	23.621582551359896
82-83	22.881257951687545	26.26048194143212	27.87816228917653	22.98009781770381
84-85	23.05268742793462	26.511974503242868	26.84542100024774	23.58991706857477
86-87	21.96909998126625	27.11673941840064	27.22923044139406	23.684930158939046
88-89	22.637942290095186	26.113218022556	27.204066851071833	24.04477283627698
90-91	22.45095451852492	26.218013059693234	27.250316528260253	24.080715893521592
92-93	22.57566018079639	26.44898833695919	27.16133153434907	23.81401994789535
94-95	21.709063585740218	26.323974005236444	27.67661266900035	24.290349740022986
96-97	22.040376311573358	26.301591013549626	27.29030772698501	24.36772494789201
98-99	21.592839582386777	26.672459025233785	27.214464423475874	24.520236968903568
100	22.120023524713222	26.232566435301685	26.679751691452473	24.967658348532616
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	573.0
1	410.0
2	365.0
3	466.5
4	376.0
5	366.0
6	665.0
7	1372.5
8	2314.5
9	3004.0
10	2917.5
11	2747.5
12	3255.0
13	4023.5
14	5024.5
15	6331.0
16	7652.5
17	9089.0
18	10495.5
19	11612.5
20	12989.0
21	14342.5
22	15836.5
23	17801.5
24	20204.0
25	23729.0
26	29093.5
27	35821.0
28	45772.0
29	65156.0
30	93113.5
31	122240.0
32	162635.0
33	206531.5
34	244387.0
35	281912.5
36	320737.0
37	379407.5
38	406307.0
39	391656.5
40	369133.0
41	345039.0
42	343892.5
43	362782.5
44	392845.5
45	453643.0
46	506530.0
47	540154.0
48	567452.0
49	560020.5
50	539404.0
51	542137.0
52	539147.5
53	514789.0
54	495792.0
55	440380.0
56	385880.0
57	324220.5
58	257801.5
59	236650.5
60	196288.5
61	142967.5
62	108893.5
63	88141.5
64	64490.5
65	37498.0
66	25163.0
67	19294.0
68	14919.0
69	11425.0
70	9134.5
71	7152.5
72	5361.0
73	4080.0
74	3101.0
75	2163.5
76	1455.5
77	938.5
78	652.0
79	435.5
80	302.5
81	224.5
82	146.5
83	83.0
84	59.5
85	63.0
86	47.0
87	22.0
88	11.0
89	6.5
90	3.5
91	1.5
92	1.0
93	1.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.35968765043157286
2	5.231527697035631E-4
3	0.002084562574664967
4	0.0013441001929306929
5	0.004619841381689927
6	0.0
7	5.6339529044999104E-5
8	2.977946535235667E-4
9	0.0029859950393849527
10-11	5.2315276970356314E-5
12-13	4.426677282107073E-5
14-15	2.173096120307108E-4
16-17	1.0463055394071263E-4
18-19	9.577719937649849E-4
20-21	1.6901858713499733E-4
22-23	1.6097008298571173E-4
24-25	0.0
26-27	0.0020000532810974686
28-29	0.011119008482238037
30-31	0.0033924444989238745
32-33	0.013972203203159778
34-35	0.011135105490536609
36-37	0.012873582386782297
38-39	0.0126482242706023
40-41	0.013811233120174068
42-43	0.006370391034159543
44-45	0.0014125124781996204
46-47	0.005887480785202406
48-49	0.0125918847415573
50-51	0.0037304816731938695
52-53	0.005718462198067409
54-55	0.005062509109900634
56-57	2.6157638485178154E-4
58-59	5.553467863007054E-4
60-61	4.104737116135649E-4
62-63	0.0010583782956310546
64-65	3.541341825685658E-4
66-67	0.0013964154699010493
68-69	0.001537264292513547
70-71	9.094809688692712E-4
72-73	0.0029859950393849527
74-75	0.008116916434554514
76-77	0.003939742781075294
78-79	0.001678113115126045
80-81	5.432740300767771E-4
82-83	2.1328535995606806E-4
84-85	0.0015050702759164046
86-87	0.004559477600570285
88-89	0.0017384768962456865
90-91	2.2535811617999642E-4
92-93	0.0020161502893960392
94-95	0.003199280399341021
96-97	0.0018551802064103277
98-99	0.002044320053918539
100	0.002317969194994249
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2424669E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.679574660211884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.06165858061486	36.38138799355563
2	15.928523910760031	16.782157291677066
3	6.364992604894258	10.059153094236708
4	3.104173238910194	6.541061035896052
5	1.7146514984374215	4.5163555814089165
6	1.0483091510778775	3.3134688114714232
7	0.6744376633807433	2.48703624692073
8	0.4576822109075902	1.9288403360125792
9	0.32458927189343045	1.5389302304352406
>10	1.256224574414432	10.61724579508578
>50	0.04000226147432891	1.4420579926509536
>100	0.022236133498097853	2.264242149107378
>500	0.0016865893583436543	0.6232674139319491
>1k	7.282717119186007E-4	0.5742477422467627
>5k	2.080773327711317E-5	0.072301561851429
>10k+	8.323093310845268E-5	0.8582467235114214
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28864	0.2323120237649792	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24397	0.19635935573012048	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13901	0.11188225617921893	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.829102489571352E-5
2	0.0	0.0	0.0	0.0	4.829102489571352E-5
3	0.0	0.0	0.0	0.0	4.829102489571352E-5
4	0.0	0.0	0.0	0.0	6.438803319428469E-5
5	0.0	0.0	0.0	0.0	6.438803319428469E-5
6	0.0	0.0	0.0	0.0	8.853354564214146E-5
7	0.0	0.0	0.0	0.0	9.658204979142704E-5
8	0.0	0.0	0.0	0.0	1.207275622392838E-4
9	0.0	0.0	0.0	8.048504149285586E-6	1.6097008298571173E-4
10-11	0.0	0.0	0.0	1.6097008298571173E-5	1.770670912842829E-4
12-13	0.0	0.0	0.0	1.6097008298571173E-5	2.53527880702496E-4
14-15	0.0	0.0	0.0	2.0121260373213966E-5	3.2194016597142345E-4
16-17	0.0	0.0	0.0	2.414551244785676E-5	3.621826867178514E-4
18-19	0.0	0.0	0.0	4.426677282107073E-5	3.70231190867137E-4
20-21	0.0	0.0	0.0	8.048504149285588E-5	4.0242520746427935E-4
22-23	0.0	0.0	0.0	1.891398475082113E-4	4.829102489571352E-4
24-25	0.0	0.0	0.0	4.708374927332068E-4	5.593710383753482E-4
26-27	0.0	0.0	0.0	0.001150936093347839	5.955893070471334E-4
28-29	0.0	0.0	0.0	0.0036862149003727986	5.955893070471334E-4
30-31	0.0	0.0	0.0	0.014302191873280487	6.076620632710617E-4
32-33	0.0	0.0	0.0	0.037892357534836545	6.720500964653466E-4
34-35	0.0	0.0	0.0	0.0751851015105513	7.00219860987846E-4
36-37	0.0	0.0	0.0	0.13845841688015995	7.00219860987846E-4
38-39	0.0	0.0	0.0	0.23315711670065417	7.485108858835596E-4
40-41	0.0	0.0	0.0	0.33399280093497863	7.565593900328452E-4
42-43	0.0	0.0	0.0	0.43860323361531806	7.887534066299875E-4
44-45	0.0	0.0	0.0	0.5646991481221753	8.209474232271298E-4
46-47	0.0	0.0	0.0	0.7154436065862197	8.571656918989149E-4
48-49	0.0	0.0	0.0	0.8796813822565415	9.014324647199857E-4
50-51	0.0	0.0	0.0	1.0514324365502212	9.215537250931997E-4
52-53	0.0	0.0	0.0	1.217907696374044	9.859417582874844E-4
54-55	0.0	0.0	0.0	1.4001459515742432	0.001014111522809984
56-57	0.0	0.0	0.0	1.614276404465986	0.001034232783183198
58-59	0.0	0.0	0.0	1.8598523630689878	0.0010463055394071264
60-61	0.0	0.0	0.0	2.1144587433274884	0.0010463055394071264
62-63	0.0	0.0	0.0	2.373061205896109	0.0010945965643028397
64-65	0.0	0.0	0.0	2.6385894062851896	0.0010986208163774826
66-67	0.0	0.0	0.0	2.93679453352037	0.001110693572601411
68-69	0.0	0.0	0.0	3.263595995997962	0.0011187420767506966
70-71	0.0	0.0	0.0	3.6222131953776797	0.001146911841273196
72-73	0.0	0.0	0.0	3.9838405353092305	0.0013078819242589078
74-75	0.0	0.0	0.0	4.341294725839377	0.0013360516887814073
76-77	0.0	0.0	0.0	4.7408747870868835	0.0013400759408560502
78-79	0.0	0.0	0.0	5.171184037176362	0.0013521486970799787
80-81	0.0	0.0	0.0	5.6322305246119635	0.0013762942095278353
82-83	0.0	0.0	0.0	6.104387972025654	0.0014648277551699768
84-85	0.0	0.0	0.0	6.565824811912494	0.0014728762593192624
86-87	0.0	0.0	0.0	7.054787535989893	0.0015050702759164046
88	0.0	0.0	0.0	7.441051347122406	0.001537264292513547
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	22220	0.0	46.823215	1
GTATCAA	39565	0.0	40.35035	1
CAACGCA	45230	0.0	33.708237	5
ATCAACG	46100	0.0	33.053032	3
GTGGTAT	7040	0.0	32.424442	1
TATCAAC	50045	0.0	31.761292	2
GTACTAG	10420	0.0	31.049612	1
AACGCAG	51725	0.0	30.427898	6
TCAACGC	54570	0.0	28.318033	4
TAGGCAT	13415	0.0	27.957218	5
ACGCAGA	55050	0.0	27.685263	7
GGGCACC	11535	0.0	27.053503	94
CGCAGAG	56225	0.0	27.014748	8
CTAGGCA	15020	0.0	25.783539	4
GCAGAGT	60525	0.0	24.777231	9
CTGTGCG	14025	0.0	24.360844	9
CGTTAAC	2760	0.0	23.923174	1
TAGTACT	14630	0.0	23.836628	4
GTACATG	57335	0.0	23.673952	1
GCATCGT	13085	0.0	23.201593	8
>>END_MODULE