FastQCFastQC Report
Thu 26 May 2016
ERR580053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4337881
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92900.040.594911
GGTATCA54350.039.3663181
CAACGCA107000.033.5708545
ATCAACG111850.032.0746083
TATCAAC118750.031.2821332
AACGCAG124350.029.9296576
TCAACGC123700.029.34344
GTACTAG26600.027.2240051
GTACATG200200.026.330221
ACGCAGA139200.025.4879597
TACATGG203650.025.421772
ACATGGG207600.024.4380723
CGCAGAG144850.024.3640128
TAGTACT30900.023.7371674
GCATCGT29150.022.8916538
TAGGCAT34350.022.44765
CATGGGG167500.022.2317244
CTAGTAC35550.021.6908743
CTAGGCA38350.020.9649374
GCAGAGT165150.020.888879
Produced by FastQC (version 0.11.5)