FastQCFastQC Report
Thu 26 May 2016
ERR580053_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580053_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4337881
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57800.041.28441
GTATCAA97300.038.386251
CAACGCA109950.032.786055
GTACTAG25400.032.1200141
ATCAACG114150.031.5811863
TATCAAC120300.030.981082
AACGCAG125400.029.307126
TCAACGC126800.028.7631514
GTACATG200850.025.3345361
TACATGG204650.024.8946762
ACGCAGA143750.024.8467547
TAGGCAT39000.024.222585
ACATGGG207650.023.5628493
CGCAGAG151550.023.3821478
GCATCGT33800.021.8299068
CTAGGCA42950.021.776294
CATGGGG167600.020.8918194
GTGGTAT28300.020.3299711
GCAGAGT171600.020.2667169
ATGGGGG113450.019.8850045
Produced by FastQC (version 0.11.5)