FastQCFastQC Report
Thu 26 May 2016
ERR580052_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580052_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7538152
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220130.29202117442046804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185610.24622745733967688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105620.14011391651428626No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122200.050.913891
GTATCAA218950.039.9284171
CAACGCA249800.033.6640625
ATCAACG252050.033.474113
TATCAAC271750.031.7984052
AACGCAG272050.031.2926126
TCAACGC278650.030.4829064
GTGGTAT46200.029.722721
GTACTAG44600.028.25841
ACGCAGA297600.027.9903377
CGCAGAG300750.027.587828
GCATCGT40950.027.5352928
GTACATG258000.025.6461431
TGGTATC52100.025.3570062
CTGTGCG57450.024.8644779
TACATGG261700.024.4682432
GCAGAGT333900.024.2614429
ACATGGG256950.023.9637053
GGGCACC52150.022.89342194
CTAGTAC69150.022.2273083
Produced by FastQC (version 0.11.5)