FastQCFastQC Report
Thu 26 May 2016
ERR580052_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580052_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7538152
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195750.2596790300858884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169500.22485617164525207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100930.13389223247289256No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA122150.052.3472141
GTATCAA220900.039.3114741
CAACGCA254500.033.021615
ATCAACG256000.032.792273
TATCAAC276650.031.2547842
AACGCAG280650.030.4282286
TCAACGC281950.029.9906734
ACGCAGA304850.027.4731397
CGCAGAG311050.026.8647358
GTACTAG44900.026.8066021
GTGGTAT44650.026.6407971
GGGCACC49300.024.690494
GTACATG260150.024.615091
CTGTGCG54150.024.3872579
GCAGAGT344450.023.8626549
TACATGG263500.023.7678032
ACATGGG256050.023.4237233
TGGTATC50250.023.392112
TAGTACT64050.022.8227714
CTAGTAC64450.022.6812763
Produced by FastQC (version 0.11.5)