FastQCFastQC Report
Thu 26 May 2016
ERR580051_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580051_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11107045
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA193100.037.9725841
CAACGCA178300.037.294575
ATCAACG194650.034.2622873
TAGTACT113550.032.141144
TATCAAC232250.031.22792
CTGTGCG120900.030.8671919
GTACATG394400.029.336241
TACATGG395250.028.7857862
GGGCACC112900.028.3495694
AACGCAG254450.028.224856
ACGCAGA235000.028.1398227
GTACTAG92900.027.9466421
GTACTGT136450.027.8322686
ACATGGG395750.027.7141883
CTAGTAC140650.027.1863
ACTGTGC147550.026.756268
CGCAGAG247050.026.747668
GGTATCA113100.026.6979981
TACTGTG154450.026.2918367
GAGTACA284100.026.2896961
Produced by FastQC (version 0.11.5)