FastQCFastQC Report
Thu 26 May 2016
ERR580051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11107045
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA193700.036.7931061
CAACGCA185650.035.4940875
ATCAACG199200.033.1270453
TAGTACT124650.030.7304064
GGGCACC113550.030.05393294
CTGTGCG129700.029.8950549
TATCAAC236750.029.8400362
GTACATG391250.028.6930541
GGTATCA113650.028.541481
TACATGG390350.028.2972352
AACGCAG254950.027.8912076
ACATGGG388500.027.3777963
CTAGTAC148300.027.2560063
GTACTGT144100.027.2336546
ACGCAGA243100.027.066157
CTAACGC49200.027.0349943
ACTGTGC157650.026.9201348
TCTAACG49600.026.9133032
TAACGCC51500.025.918754
TACTGTG165400.025.9144977
Produced by FastQC (version 0.11.5)