FastQCFastQC Report
Thu 26 May 2016
ERR580050_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580050_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7586361
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123230.16243624578371632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103350.13623132355552287No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102250.048.561861
GTATCAA173150.038.9965931
ATCAACG202850.032.127733
CAACGCA201750.032.089125
TATCAAC217150.030.7707142
AACGCAG222000.029.763866
TCAACGC220800.029.6004944
GTACTAG37650.028.8497261
ACGCAGA246850.026.2153027
CGCAGAG254000.025.4025068
GTACATG263100.025.2174321
GTGGTAT42600.024.504061
TACATGG266250.024.1071762
ACATGGG269400.022.9519713
GCAGAGT286400.021.9241059
GCATCGT38400.021.5387868
CATGGGG210900.021.4923384
CTAACGC17050.021.2352893
TCTAACG17700.020.9873262
TAACGCC17250.020.7160874
Produced by FastQC (version 0.11.5)