FastQCFastQC Report
Thu 26 May 2016
ERR580050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7586361
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109200.1439425305492317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99830.13159141780888095No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103450.047.1852041
GTATCAA176150.039.0900461
CAACGCA200550.032.995615
ATCAACG203300.032.596163
TATCAAC221250.030.69372
AACGCAG221050.030.5082636
TCAACGC221000.030.2615814
ACGCAGA245900.026.8136067
CGCAGAG254050.025.8239278
GTACTAG37350.025.3805751
GTACATG256850.024.6599641
TACATGG262150.023.6998862
CTGTGCG51600.023.5888189
TAGTACT54150.023.4338874
GTGGTAT48600.023.3871211
CTAGTAC53650.023.3021853
ACATGGG262800.022.6766363
GCAGAGT289100.022.4004759
CATGGGG204750.021.117524
CCTAGTA62600.020.119872
Produced by FastQC (version 0.11.5)