FastQCFastQC Report
Thu 26 May 2016
ERR580049_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580049_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032860
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87350.12420267145940628No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114700.042.6356051
GTATCAA206850.040.395631
CAACGCA221050.035.8567285
ATCAACG236650.033.537553
TATCAAC251350.033.0380442
AACGCAG271500.030.459376
GTACTAG62900.029.5290151
TACCCCG7500.028.2068675
ACGCAGA280500.027.9902137
CGCAGAG284600.027.4547128
CGTTAAC20650.027.0975111
GGGCACC65150.026.97452494
GTGGTAT38150.026.86981
GCATCGT63150.026.859228
TCAACGC301800.026.7951454
TAGGCAT91500.025.6379385
AAGCTAA55550.025.4653769
GTATAAG73150.025.1341921
GCAGAGT307850.025.0974929
CTGTGCG82150.024.6568379
Produced by FastQC (version 0.11.5)