FastQCFastQC Report
Thu 26 May 2016
ERR580049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7032860
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77730.11052402578751745No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117750.044.094761
GTATCAA206450.039.0608631
CAACGCA225050.033.537935
TACCCCG7550.032.3687635
ATCAACG237200.031.7416783
TATCAAC255750.031.2766192
GTACTAG65150.029.9672261
AACGCAG274850.028.7941136
GGGCACC66850.027.1366194
GTGGTAT41100.027.0788881
TAGGCAT89450.026.5326585
ACGCAGA284350.026.3115967
CGCAGAG287150.025.875378
TCAACGC304250.025.05514
AAGCTAA55550.024.9569939
CGTTAAC24000.024.9547421
TAGTACT84450.024.5979234
CTAGGCA100750.024.3034844
CTGTGCG86200.023.9337929
GCAGAGT314550.023.2324479
Produced by FastQC (version 0.11.5)