FastQCFastQC Report
Thu 26 May 2016
ERR580048_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580048_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6893250
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173520.2517245131106517No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139120.20182062162260184No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76530.11102165161571101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA118750.050.099911
GTATCAA216000.040.9122161
CAACGCA244600.034.6521345
ATCAACG252000.033.6711463
TATCAAC269250.032.4100152
GTACTAG53700.031.6164361
AACGCAG276600.031.338596
TCAACGC287150.029.7465654
TAGGCAT64700.029.2813765
GTGGTAT43150.029.1011711
ACGCAGA296150.028.4928677
GCATCGT57250.028.4766738
CGCAGAG301650.027.7704758
GTACATG343900.027.6795081
TACATGG350000.026.9475842
ACATGGG346700.026.2230913
CTAGGCA72900.026.0522084
TAGTACT66750.025.3537354
CTGTGCG66300.025.1599649
GCAGAGT327950.025.0311289
Produced by FastQC (version 0.11.5)