FastQCFastQC Report
Thu 26 May 2016
ERR580048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6893250
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156620.2272077757226272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132090.1916222391469916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73490.10661154027490662No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117000.050.556161
GTATCAA214850.040.749631
CAACGCA250950.033.7492525
ATCAACG255300.033.1560363
TATCAAC273950.031.8038942
GTACTAG51550.031.6504651
AACGCAG282350.030.7432986
GTGGTAT42350.030.5322361
TCAACGC291650.029.4746254
TAGGCAT62650.027.75735
ACGCAGA305500.027.5675497
CGCAGAG311950.026.9374858
TGGTATC47600.026.5718332
GTACATG344400.026.1447771
GGGCACC62450.025.58635394
TACATGG348900.025.5109022
TAGTACT68400.025.42394
ACATGGG344100.025.0230643
CTAGGCA71700.024.974824
GCATCGT61500.024.9124818
Produced by FastQC (version 0.11.5)