FastQCFastQC Report
Thu 26 May 2016
ERR580047_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580047_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3742477
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82280.21985439055470482No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66210.17691491490796069No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61850.051.6231841
GTATCAA117650.042.6468731
CAACGCA135350.035.979855
ATCAACG136750.035.61153
TATCAAC145650.034.278232
AACGCAG149900.033.194526
TCAACGC154650.031.7636244
GTGGTAT23100.029.5168971
ACGCAGA164250.029.352367
CGCAGAG165550.028.9803358
GCAGAGT182450.025.9875779
GTACTAG23150.024.3750211
TGGTATC27400.023.5057622
CGTTAAC6650.023.3349591
GTACATG179500.023.236671
TACATGG179700.022.7863312
GCTATTG29850.022.6686178
GCATCGT21850.022.581088
ACATGGG177800.022.3135433
GAGTACT79050.022.29417412-13
Produced by FastQC (version 0.11.5)