FastQCFastQC Report
Thu 26 May 2016
ERR580047_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580047_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3742477
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72790.1944968532872747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60650.16205844418015128No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61700.052.882951
GTATCAA116950.041.9702531
CAACGCA137950.034.344595
ATCAACG138550.034.0945363
TATCAAC145750.033.1940162
AACGCAG152050.031.6839646
TCAACGC154250.030.8067194
GTGGTAT26000.028.3901481
ACGCAGA167400.028.1609657
CGCAGAG168150.028.0070348
GCAGAGT186500.024.872379
GTACTAG24700.023.7932851
TGGTATC30100.023.5864942
CGTTAAC7950.023.0642221
GTACATG175700.022.63811
TACATGG175700.022.3978672
GAGTACT82750.022.26263612-13
ACATGGG177050.021.6099473
GCTATTG32200.021.310488
CAGAGTA184800.020.6499210-11
Produced by FastQC (version 0.11.5)