FastQCFastQC Report
Thu 26 May 2016
ERR580046_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580046_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6012581
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120670.20069584093752751No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97910.1628418810490869No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88950.048.157291
GTATCAA166800.043.1587371
CAACGCA185450.037.518465
ATCAACG189200.036.9049073
TATCAAC202900.035.1795432
AACGCAG207950.034.3246886
TCAACGC213100.032.8745544
ACGCAGA226950.030.4978667
CGCAGAG229500.030.0561268
GTACTAG40350.026.685881
GCAGAGT254700.026.5157669
GTGGTAT34950.026.2347561
GCATCGT37900.025.0438928
TAGGCAT52800.023.6841495
GTACATG248300.023.2736641
TACATGG245750.022.8269232
ACATGGG245600.022.0547893
GAGTACT120100.021.39335612-13
CTAGGCA59150.021.3028224
CAGAGTA258100.020.60616310-11
Produced by FastQC (version 0.11.5)