FastQCFastQC Report
Thu 26 May 2016
ERR580046_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580046_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6012581
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107470.17874187474563752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91730.1525634332410657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93050.050.5926171
GTATCAA162350.039.1661221
CAACGCA183000.033.2066155
ATCAACG185300.032.896993
TATCAAC201200.031.4634252
AACGCAG207950.030.012236
TCAACGC211100.029.0092834
GTGGTAT35400.026.9166561
ACGCAGA228250.026.622387
CGCAGAG231300.026.1699568
GTACTAG38600.025.2962421
GCAGAGT257550.023.1012179
GTACATG252750.022.8062041
TACATGG252400.022.3624822
CTGTGCG48900.021.9123389
TGGTATC43050.021.6151562
TAGGCAT50400.021.540645
CGTTAAC10800.021.4015541
ACATGGG252900.021.3531933
GGGCACC45500.021.17358294
Produced by FastQC (version 0.11.5)