##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580046_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6012581
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.53433725050856	34.0	31.0	34.0	31.0	34.0
2	32.73350313284761	34.0	31.0	34.0	31.0	34.0
3	32.81622750695583	34.0	31.0	34.0	31.0	34.0
4	36.28497761610197	37.0	37.0	37.0	35.0	37.0
5	36.21877909004469	37.0	37.0	37.0	35.0	37.0
6	36.24807216734377	37.0	37.0	37.0	35.0	37.0
7	36.21871838400181	37.0	37.0	37.0	35.0	37.0
8	36.210300867464404	37.0	37.0	37.0	35.0	37.0
9	37.99445080240915	39.0	38.0	39.0	35.0	39.0
10-11	37.94525554000853	39.0	38.0	39.0	35.0	39.0
12-13	37.90912272117416	39.0	38.0	39.0	35.0	39.0
14-15	39.30680759228025	41.0	39.0	41.0	36.0	41.0
16-17	39.29695816156156	41.0	39.0	41.0	36.0	41.0
18-19	39.29952494610883	41.0	39.0	41.0	36.0	41.0
20-21	39.28525195752041	41.0	39.0	41.0	36.0	41.0
22-23	39.21582703002255	41.0	39.0	41.0	36.0	41.0
24-25	39.134661054878094	40.5	39.0	41.0	35.0	41.0
26-27	38.99886995285385	40.0	38.5	41.0	35.0	41.0
28-29	38.89912310536856	40.0	38.0	41.0	35.0	41.0
30-31	38.792244295752525	40.0	38.0	41.0	35.0	41.0
32-33	38.65441954129183	40.0	38.0	41.0	35.0	41.0
34-35	38.628757600105516	40.0	38.0	41.0	34.5	41.0
36-37	38.677960180494864	40.0	38.0	41.0	35.0	41.0
38-39	38.586481911844515	40.0	38.0	41.0	35.0	41.0
40-41	38.464870078257576	40.0	38.0	41.0	34.0	41.0
42-43	38.441015763446686	40.0	38.0	41.0	34.0	41.0
44-45	38.35136283403084	40.0	38.0	41.0	34.0	41.0
46-47	38.16510047848004	40.0	38.0	41.0	33.5	41.0
48-49	37.95884845459878	40.0	37.0	41.0	33.0	41.0
50-51	37.790713089769596	40.0	37.0	41.0	33.0	41.0
52-53	37.6165190123842	40.0	36.5	41.0	33.0	41.0
54-55	37.42484966106902	40.0	36.0	41.0	33.0	41.0
56-57	37.21139024987772	39.0	35.5	41.0	32.0	41.0
58-59	36.98162062182614	39.0	35.0	41.0	32.0	41.0
60-61	36.73373689269218	39.0	35.0	40.5	31.5	41.0
62-63	36.45365251628211	38.0	35.0	40.0	31.0	41.0
64-65	36.17317122214237	37.5	35.0	40.0	31.0	41.0
66-67	35.859218195979395	37.0	35.0	40.0	31.0	41.0
68-69	35.52275054589701	36.5	35.0	39.0	30.5	41.0
70-71	35.21163573513604	36.0	34.5	39.0	30.0	41.0
72-73	34.87012865190506	35.5	34.0	38.5	30.0	40.0
74-75	34.520179520242635	35.0	34.0	37.5	29.5	39.5
76-77	33.2793104991018	34.5	32.5	36.0	28.0	39.0
78-79	33.90507737026744	35.0	34.0	36.5	29.0	39.0
80-81	33.83582075651039	35.0	34.0	36.0	29.0	38.0
82-83	33.619424087592336	35.0	34.0	36.0	29.0	37.0
84-85	33.39613944494053	35.0	34.0	35.0	29.0	37.0
86-87	33.22258760422521	35.0	34.0	35.0	29.0	36.5
88-89	33.06161006396421	35.0	34.0	35.0	29.0	36.0
90-91	32.89808819207592	35.0	33.0	35.0	29.0	36.0
92-93	32.78287095009614	35.0	33.0	35.0	29.0	35.5
94-95	32.69047834864928	35.0	33.0	35.0	29.0	35.0
96-97	32.59639637287215	35.0	33.0	35.0	28.5	35.0
98-99	32.52883345771142	35.0	33.0	35.0	27.0	35.0
100	31.25165232701231	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.15994851486707518
1101	2	0.08305323536993114
1101	3	0.015466232859672857
1101	4	0.044457895054250685
1101	5	-0.0073504503823329514
1101	6	0.010079483126560262
1101	7	0.01124789230762957
1101	8	-0.0011619880448918707
1101	9	0.02103157664078026
1101	10-11	-0.010917129978210482
1101	12-13	-0.007129304614004184
1101	14-15	-0.05984514939763841
1101	16-17	-0.06696513161956119
1101	18-19	-0.05305709256881386
1101	20-21	-0.03968259248312478
1101	22-23	-0.04569472910677774
1101	24-25	-0.08033194822477441
1101	26-27	-0.10686431067862401
1101	28-29	-0.09015541651181991
1101	30-31	-0.129747453728271
1101	32-33	-0.10881380994023715
1101	34-35	-0.15727234914754007
1101	36-37	-0.11881136293278871
1101	38-39	-0.09077071158088046
1101	40-41	-0.04693767269492355
1101	42-43	-0.07100710324955628
1101	44-45	-0.14564884029938696
1101	46-47	-0.1207547325385292
1101	48-49	-0.17350214784668339
1101	50-51	-0.26083366801344
1101	52-53	-0.18130269191589576
1101	54-55	-0.15805959523984114
1101	56-57	-0.2043592926340878
1101	58-59	-0.18901265745862617
1101	60-61	-0.20363019798905668
1101	62-63	-0.18471673696475222
1101	64-65	-0.2673551813249375
1101	66-67	-0.23889346071047157
1101	68-69	-0.28297361272173305
1101	70-71	-0.32296749737057695
1101	72-73	-0.28074340753737204
1101	74-75	-0.29991407285865535
1101	76-77	-0.2626552046881301
1101	78-79	-0.15930781406187577
1101	80-81	-0.15023252735995385
1101	82-83	-0.13828122299849355
1101	84-85	-0.145232619246066
1101	86-87	-0.15117915621189582
1101	88-89	-0.11504869524021899
1101	90-91	-0.1806998595864684
1101	92-93	-0.24353051970287964
1101	94-95	-0.19536418722055515
1101	96-97	-0.17540061733859602
1101	98-99	-0.20436982057400144
1101	100	-0.19135157390799407
1102	1	0.09435850860493389
1102	2	0.09104924258477354
1102	3	0.0537748119954955
1102	4	0.036681544545515976
1102	5	0.015975175698159205
1102	6	0.04055674405453402
1102	7	0.03877102744681338
1102	8	0.038911391424910846
1102	9	0.020394818396965775
1102	10-11	0.058527519636157876
1102	12-13	0.009754824332617318
1102	14-15	0.036670396238399405
1102	16-17	0.031184565710191237
1102	18-19	-0.006267190140498258
1102	20-21	0.01517875724952944
1102	22-23	0.020679623938924863
1102	24-25	0.040123368441626894
1102	26-27	0.05455567037437703
1102	28-29	0.00358503639632346
1102	30-31	0.03239500199516954
1102	32-33	0.009230421127476518
1102	34-35	0.04339122391346706
1102	36-37	0.07454969510175147
1102	38-39	0.08019133019885061
1102	40-41	0.08593175359644079
1102	42-43	0.052210380439440485
1102	44-45	0.0861377704896995
1102	46-47	0.04284393903080996
1102	48-49	0.0016888742742011686
1102	50-51	-0.01590742214137464
1102	52-53	0.004658065321699212
1102	54-55	-0.015860111197596893
1102	56-57	0.02361225475748796
1102	58-59	-0.08472696993420215
1102	60-61	-0.06372220331990519
1102	62-63	-0.10034196109059934
1102	64-65	-0.03151169208030069
1102	66-67	-0.09623055132956182
1102	68-69	-0.04114793044252707
1102	70-71	-0.07135858157766961
1102	72-73	-0.026056300593886306
1102	74-75	-0.01527310238706292
1102	76-77	-0.007194509001514859
1102	78-79	0.00627649204452041
1102	80-81	0.0444481033502413
1102	82-83	0.04081793830253133
1102	84-85	0.004391197235683819
1102	86-87	0.007589770713714472
1102	88-89	0.0039003619593742656
1102	90-91	0.008590618209687761
1102	92-93	0.038689169116786104
1102	94-95	-0.0054038206978717085
1102	96-97	-0.053159599767127474
1102	98-99	-0.05856237879319792
1102	100	0.0796306726491629
1103	1	0.08556338329501045
1103	2	0.0537582404889676
1103	3	-0.005832379565305246
1103	4	-0.0038278104137958735
1103	5	-0.08387473663525924
1103	6	-0.021017064775705308
1103	7	-0.006050516160861719
1103	8	-0.011882665609810772
1103	9	-0.039970091240235206
1103	10-11	-0.07579591675914799
1103	12-13	-0.08356350113949418
1103	14-15	-0.02666357579282419
1103	16-17	-0.04093274279262715
1103	18-19	-0.01617007238652235
1103	20-21	-0.08291575902836001
1103	22-23	-0.11472774724682466
1103	24-25	-0.12418747725703838
1103	26-27	-0.11290471016497605
1103	28-29	-0.13195252811224378
1103	30-31	-0.14643993699243651
1103	32-33	-0.0996571297614608
1103	34-35	-0.17456714812292518
1103	36-37	-0.07963595096081377
1103	38-39	-0.10600235650337453
1103	40-41	-0.05155748840950025
1103	42-43	-0.050721403148784816
1103	44-45	-0.08708167487859697
1103	46-47	-0.06747276403638836
1103	48-49	-0.039028483057215624
1103	50-51	-0.054748371263464435
1103	52-53	-0.022061852849638797
1103	54-55	-0.005295179742901723
1103	56-57	-0.02109134614045871
1103	58-59	-0.08994911293640229
1103	60-61	-0.06100370806678512
1103	62-63	-0.08244278314747788
1103	64-65	-0.14202534208827444
1103	66-67	-0.14203820138381928
1103	68-69	-0.10096095131767413
1103	70-71	-0.15332128136724066
1103	72-73	-0.1570295745911494
1103	74-75	-0.18029628999985192
1103	76-77	-0.1888063713326602
1103	78-79	-0.08275046538886954
1103	80-81	-0.023137619160500833
1103	82-83	-0.04669691047274682
1103	84-85	0.0022984203818765536
1103	86-87	-0.010995957664285072
1103	88-89	0.031634677072958084
1103	90-91	0.0011699383490082482
1103	92-93	-0.03515994418329882
1103	94-95	-0.05959424477117636
1103	96-97	-0.03834625890922183
1103	98-99	-0.0734839650905883
1103	100	-0.01879518061011254
1104	1	0.09476649694190797
1104	2	0.07867679627177182
1104	3	0.05040434834581475
1104	4	0.03176211184236877
1104	5	0.03936448040923324
1104	6	0.07477453921516286
1104	7	0.0196531739625172
1104	8	0.07067794999155552
1104	9	0.038313452829598305
1104	10-11	0.007529724159809348
1104	12-13	0.013156433758609865
1104	14-15	0.05586611416347864
1104	16-17	0.03680808495251853
1104	18-19	0.022536039804499808
1104	20-21	-0.07288928517869664
1104	22-23	-0.05854349800276282
1104	24-25	-0.05564877375206123
1104	26-27	-0.03931060630166172
1104	28-29	-0.06904542067433539
1104	30-31	-0.06574416586649079
1104	32-33	-0.09827626561470026
1104	34-35	-0.11803477478864721
1104	36-37	-0.08029819831313034
1104	38-39	-0.0063459293226912905
1104	40-41	-0.051443465970606894
1104	42-43	-0.05668069339440507
1104	44-45	-0.06837391920279856
1104	46-47	0.0023742159252932993
1104	48-49	-0.02002378503323854
1104	50-51	-0.06983329391378135
1104	52-53	-0.06531625434575261
1104	54-55	-0.09461940639854305
1104	56-57	-0.04636243823262021
1104	58-59	-0.015500362225495223
1104	60-61	-0.06579275746299373
1104	62-63	-0.05369684118576146
1104	64-65	-0.07801857593685213
1104	66-67	-0.06538764635873662
1104	68-69	-0.053899245924924344
1104	70-71	-0.10675799629306937
1104	72-73	-0.102693309797246
1104	74-75	-0.1855101558771608
1104	76-77	-0.1946627499093978
1104	78-79	-0.06898487648382456
1104	80-81	-0.04712597218551906
1104	82-83	0.023074018875377078
1104	84-85	0.06571456420503097
1104	86-87	-0.02132497604339534
1104	88-89	0.005863185848781427
1104	90-91	0.061694220154713264
1104	92-93	-0.011267269461825435
1104	94-95	-0.026684759013441806
1104	96-97	-0.10982811313655816
1104	98-99	-0.09132085567087245
1104	100	-0.0020019804120607887
1105	1	0.13023623693005248
1105	2	0.09870380938020418
1105	3	0.07484889878126921
1105	4	0.04194613276833792
1105	5	0.010226471818405969
1105	6	0.005878481037420613
1105	7	-0.004521651311655717
1105	8	0.008293965192734731
1105	9	0.060139639585592874
1105	10-11	-0.01134703131035053
1105	12-13	-0.008746824959310118
1105	14-15	0.07392919427954325
1105	16-17	0.02861881512149722
1105	18-19	-0.02173674163660877
1105	20-21	-0.04123726449926579
1105	22-23	0.013205250926482393
1105	24-25	8.217005294497426E-4
1105	26-27	-0.002234506098069744
1105	28-29	0.016620852816721765
1105	30-31	-0.09454095877556767
1105	32-33	-0.07937085518433662
1105	34-35	-0.03341277831184186
1105	36-37	0.015668985908938282
1105	38-39	0.02258627362898835
1105	40-41	0.008958679823905413
1105	42-43	0.04964129318001653
1105	44-45	0.04846696143222573
1105	46-47	5.527388030657221E-5
1105	48-49	-0.01864638409207231
1105	50-51	0.03213419357550862
1105	52-53	0.014071925667430207
1105	54-55	-0.06197635939511059
1105	56-57	-0.020776696663084238
1105	58-59	0.02152509107033751
1105	60-61	-0.02332776218078436
1105	62-63	-0.04485315402036605
1105	64-65	-0.020671174415646476
1105	66-67	-0.007165667678457055
1105	68-69	0.04139442655780101
1105	70-71	-0.0030770196294724883
1105	72-73	0.01533828725023767
1105	74-75	0.005917917074896195
1105	76-77	0.00655900359042505
1105	78-79	0.10051602300953277
1105	80-81	0.07690898590453088
1105	82-83	0.07797873859568227
1105	84-85	0.06877400863956495
1105	86-87	0.08267777472596549
1105	88-89	0.10239249333022826
1105	90-91	0.06359738486941069
1105	92-93	-0.012417260045474166
1105	94-95	0.04835801512425775
1105	96-97	-0.056390324417137094
1105	98-99	-0.015660945023071804
1105	100	0.0356195788429261
1106	1	0.07595755772512547
1106	2	0.05808772984020294
1106	3	0.021399971635702286
1106	4	-0.01276169143665129
1106	5	-0.009851193340153941
1106	6	-0.007593148430039776
1106	7	0.024975470901949848
1106	8	0.0018738968178695359
1106	9	-0.023626344350965667
1106	10-11	-0.010158968055861806
1106	12-13	-0.014505867478845857
1106	14-15	-0.013936193571787214
1106	16-17	-0.05015512716182258
1106	18-19	-0.07281244717196955
1106	20-21	-0.0705325096963989
1106	22-23	-0.03389600168738838
1106	24-25	-0.018704101104255244
1106	26-27	-0.04068308734550641
1106	28-29	-0.022317768716284547
1106	30-31	-0.06309084352639616
1106	32-33	-0.090706388852972
1106	34-35	-0.015003143687863485
1106	36-37	0.010806252597149069
1106	38-39	-0.0754426691686021
1106	40-41	-0.025265062365690483
1106	42-43	-0.009963141373908968
1106	44-45	-0.04438265443256739
1106	46-47	-0.04845575569758864
1106	48-49	-0.11528877566726692
1106	50-51	-0.0949319560263504
1106	52-53	-0.05946331409000294
1106	54-55	-0.06089132590701496
1106	56-57	-0.12344489729562014
1106	58-59	-0.10642745611705351
1106	60-61	-0.04480720238311875
1106	62-63	-0.0634136685489537
1106	64-65	-0.11217984327912944
1106	66-67	-0.057166614460285814
1106	68-69	-0.048451254998155946
1106	70-71	-0.07573268024433588
1106	72-73	-0.08743812086179048
1106	74-75	-0.0866472607845239
1106	76-77	-0.15465086899855862
1106	78-79	-0.04132040719694885
1106	80-81	-0.05948083978433516
1106	82-83	0.016437355192508107
1106	84-85	0.016571229275449184
1106	86-87	0.028576065720223198
1106	88-89	-0.01204088356941213
1106	90-91	-0.01091465399942848
1106	92-93	-0.05815662617935402
1106	94-95	-0.05707917267981344
1106	96-97	-0.06569328965174037
1106	98-99	-0.05974485506847316
1106	100	-0.06956072714835315
1107	1	0.10929775539276676
1107	2	0.058211581743549345
1107	3	0.002668394606324398
1107	4	0.010195387161445524
1107	5	0.03049101891514283
1107	6	0.0543174791968255
1107	7	0.004162132303861199
1107	8	0.015329487723697355
1107	9	0.003656655568846645
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2315	94-95	0.006642076123547724
2315	96-97	-0.011941595478219824
2315	98-99	-0.04906686867602161
2315	100	-0.12014593766492965
2316	1	0.07173823897132081
2316	2	-0.028567523247076565
2316	3	-0.045127246917047614
2316	4	-0.18467807406113934
2316	5	-0.10215177237506623
2316	6	-0.10539859573311361
2316	7	-0.050675758758877976
2316	8	-0.03828534876023326
2316	9	-0.021618822405635285
2316	10-11	-0.09690337310008346
2316	12-13	-0.03278844278312931
2316	14-15	-0.031444305688850704
2316	16-17	-0.039839570897520105
2316	18-19	-0.034431375857593594
2316	20-21	-0.013676126700687519
2316	22-23	-0.08392781298172025
2316	24-25	-0.06008052598878777
2316	26-27	-0.032503897375669055
2316	28-29	0.023017018025548452
2316	30-31	-0.3610884883280363
2316	32-33	-0.19956480771164564
2316	34-35	-0.23356277737822495
2316	36-37	-0.5452823532962654
2316	38-39	-0.42997882102935847
2316	40-41	-0.35804602861871615
2316	42-43	-0.44427684768832165
2316	44-45	-0.30610277501760663
2316	46-47	-0.3869415740125106
2316	48-49	-0.3564637049045345
2316	50-51	-0.3949073098200344
2316	52-53	-0.3471308080016726
2316	54-55	-0.2511183254755096
2316	56-57	-0.9998956654569255
2316	58-59	-0.7430536471881979
2316	60-61	-0.46546131205008123
2316	62-63	-0.23652612440582033
2316	64-65	-0.18196066543206513
2316	66-67	-0.1124758049729877
2316	68-69	-0.21472456947606133
2316	70-71	-0.2843717106941668
2316	72-73	-0.17194884963306833
2316	74-75	-0.1361117527837763
2316	76-77	-0.18395631600225215
2316	78-79	-0.2206481028966607
2316	80-81	-0.22435519078502608
2316	82-83	-0.40088104674936886
2316	84-85	-0.21484796609514234
2316	86-87	-0.2720888030548849
2316	88-89	-0.25278938778244253
2316	90-91	-0.13932449493846377
2316	92-93	-0.14045166624197236
2316	94-95	-0.18419014183081828
2316	96-97	-0.4279684974891893
2316	98-99	-0.1846575118471776
2316	100	-0.4813247139390455
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	4.0
13	38.0
14	175.0
15	587.0
16	1444.0
17	3180.0
18	5843.0
19	9294.0
20	14474.0
21	22128.0
22	29206.0
23	30782.0
24	28721.0
25	30093.0
26	35901.0
27	43539.0
28	54773.0
29	67919.0
30	83644.0
31	105795.0
32	134044.0
33	176770.0
34	265624.0
35	405905.0
36	724808.0
37	1511421.0
38	1839119.0
39	387287.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.9080668385706	20.54226348035186	14.35827865294039	23.191391028137154
2	15.344428908414354	20.560587717651106	40.271335924692146	23.823647449242394
3	18.71417335236714	26.094032408370687	30.328323236798788	24.863471002463385
4	12.918169442418566	17.749266734137382	39.51875139813239	29.813812425311664
5	13.477153282461945	38.261819214854384	34.6161177295957	13.644909773087974
6	29.863697470354246	37.81066733238189	18.50478188984065	13.820853307423217
7	26.945271414268085	32.86708867075365	22.392517022642526	17.795122892335733
8	26.346124228656087	34.68028083887091	20.12697424290959	18.84662068956341
9	26.065376418198312	16.213374333167785	21.060305216800927	36.660944031832976
10-11	25.189041238058156	26.185585648217064	27.91058642575388	20.7147866879709
12-13	25.903339632350715	23.658171253661905	27.95120495734109	22.487284156646282
14-15	23.303441187826717	25.18319774079612	25.772064996269062	25.7412960751081
16-17	22.503705358260305	28.016990719369637	26.53872262581607	22.94058129655398
18-19	21.985184768770743	27.5867548902263	28.5550579926565	21.873002348346457
20-21	23.441576099385202	26.62250636791453	28.204992378460776	21.730925154239497
22-23	23.31996522617869	26.36728992216139	27.915139102769547	22.397605748890367
24-25	22.91123396092294	26.597412991192964	28.234338963583195	22.257014084300902
26-27	22.764111420080955	26.989524907720693	27.640407334577084	22.605956337621265
28-29	22.284210642752	27.015363175767693	28.121149548090273	22.57927663339003
30-31	23.058294755104512	26.536252335920867	28.10525600594975	22.300196903024865
32-33	22.98840938287894	26.82536957682163	27.62567092388425	22.560550116415182
34-35	23.049969984375906	26.970497860149646	27.909956121798395	22.06957603367605
36-37	22.951234841901165	26.726559262206262	27.614626761224642	22.707579134667935
38-39	22.959911965828955	26.726989025586782	27.93437325680107	22.378725751783193
40-41	23.07791345939439	26.13189344347035	28.247888458826747	22.542304638308515
42-43	22.819645752911246	26.283026922356267	27.90299392422972	22.994333400502764
44-45	23.0446422490347	26.644143587870566	27.711270495739747	22.59994366735498
46-47	22.891736601624682	26.578801896512477	27.210102269662478	23.31935923220036
48-49	23.344066792077022	26.42061828781095	27.543123602835855	22.692191317276173
50-51	23.230646124900538	26.5198585042486	27.235404009356763	23.014091361494103
52-53	22.874087838607664	26.73759541175314	27.18956573691197	23.19875101272722
54-55	22.84698007875886	26.642938547731344	27.23343040139874	23.27665097211106
56-57	23.318598918016306	26.53945257163433	26.93216333953839	23.209785170810974
58-59	22.672159518566318	26.79993144330064	27.518878663872844	23.009030374260202
60-61	22.90661790203998	26.930306666633403	27.127402576774767	23.03567285455185
62-63	23.08562093780647	26.601178987782266	26.927015172729686	23.38618490168158
64-65	22.50007026958072	26.556196787424458	27.28922909529869	23.65450384769613
66-67	22.912435887584603	26.52102641070599	27.1419675654962	23.42457013621321
68-69	22.57823216655045	26.527264781668443	27.269862772761122	23.62464027901999
70-71	22.52415710023654	26.225094045456498	27.30494112450058	23.945807729806383
72-73	22.650243272277837	26.478440635759537	27.245763296813802	23.625552795148824
74-75	22.76308373452037	26.637732575444883	27.119123753785	23.48005993624975
76-77	22.497468115890683	26.61186919327827	26.868367474058687	24.022295216772356
78-79	22.422272325531655	26.517971161915927	27.070706096300583	23.989050416251835
80-81	22.327978783712503	26.886659829302058	27.108978128333394	23.67638325865205
82-83	22.724844103725005	26.45490369636353	27.54847904927201	23.271773150639454
84-85	22.781186335557653	26.450553440418357	27.041025160827953	23.72723506319604
86-87	21.973095177307204	26.8546256385862	27.376813052564696	23.795466131541897
88-89	22.40425316051473	26.116596007963448	27.385941218833526	24.093209612688295
90-91	22.39435981383993	26.278532423960314	27.29409690618858	24.03301085601118
92-93	22.288255732639463	26.352199537109378	27.399587072493336	23.959957657757826
94-95	21.788364667212875	26.284740621874136	27.629599247680854	24.297295463232132
96-97	21.865622322482707	26.227189101546717	27.538967271159613	24.36822130481096
98-99	21.514811184990435	26.42435486801723	27.467518346359814	24.593315600632522
100	21.844555152518733	26.09416624850372	27.164535879046152	24.896742719931396
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	95.0
1	76.0
2	75.5
3	107.0
4	101.0
5	104.0
6	218.0
7	437.5
8	739.5
9	962.0
10	983.0
11	973.0
12	1099.5
13	1389.5
14	1678.5
15	2043.5
16	2434.0
17	2772.5
18	3245.0
19	3808.5
20	4368.5
21	4948.0
22	5739.5
23	6866.0
24	8228.5
25	10041.0
26	12557.5
27	15652.0
28	19570.0
29	25486.5
30	33667.0
31	43730.0
32	57614.0
33	74938.5
34	92594.5
35	111234.0
36	136327.0
37	168426.5
38	190039.0
39	199535.0
40	203634.0
41	204902.5
42	210651.0
43	221810.5
44	234539.0
45	255857.0
46	275906.0
47	288987.5
48	297063.0
49	292099.0
50	279241.0
51	268009.5
52	255698.5
53	238985.0
54	223826.5
55	199222.5
56	173043.5
57	143203.0
58	114213.0
59	99251.0
60	79722.0
61	57963.0
62	43508.5
63	33639.5
64	23874.0
65	14457.0
66	9666.0
67	6939.0
68	5070.0
69	3684.0
70	2642.5
71	1979.5
72	1440.0
73	981.5
74	737.5
75	487.5
76	301.0
77	181.0
78	109.0
79	74.5
80	48.5
81	22.5
82	15.0
83	11.5
84	6.5
85	8.0
86	5.0
87	2.0
88	1.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3534255921042893
2	5.654809473668628E-4
3	0.0020290786934928614
4	0.0015966520866829072
5	0.004906378808036017
6	0.0
7	1.6631792569613615E-5
8	3.825312291011131E-4
9	0.0033097267213531094
10-11	6.652717027845446E-5
12-13	4.989537770884085E-5
14-15	3.409517476770791E-4
16-17	7.484306656326127E-5
18-19	8.731691099047147E-4
20-21	2.6610868111381783E-4
22-23	1.995815108353634E-4
24-25	0.0
26-27	0.0021288694489105427
28-29	0.011733729657862405
30-31	0.0038336281872959383
32-33	0.015101667653209163
34-35	0.011841836309564894
36-37	0.013629754010798358
38-39	0.013712912973646426
40-41	0.015259669682620491
42-43	0.007010300568092139
44-45	0.0013554910944235097
46-47	0.006444819620725276
48-49	0.014137023684171574
50-51	0.004074789179555336
52-53	0.005821127399364765
54-55	0.005072696733732153
56-57	2.245291996897838E-4
58-59	6.569558064997378E-4
60-61	4.3242660680995403E-4
62-63	8.399055247654875E-4
64-65	3.658994365314995E-4
66-67	0.0015800202941132935
68-69	0.0018045494938030773
70-71	0.0010478029318856576
72-73	0.003026986247669678
74-75	0.00875663878790157
76-77	0.004324266068099539
78-79	0.001912656145505566
80-81	5.072696733732153E-4
82-83	1.5800202941132935E-4
84-85	0.0016631792569613613
86-87	0.0045903747492133575
88-89	0.0018461289752271111
90-91	2.245291996897838E-4
92-93	0.002419925818878781
94-95	0.003974998424137654
96-97	0.00218708072290419
98-99	0.002469821196587622
100	0.002860668321973542
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6012581.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.235431450052815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4697325411737	45.72420503219931
2	15.51716267010354	19.314346273090948
3	5.367487926382073	10.02143780704013
4	2.286568443086755	5.6922229438231895
5	1.1984171808457718	3.729200515354629
6	0.6383471584602598	2.3836686493013697
7	0.39991552498670263	1.7422240668785272
8	0.269712734577303	1.3428550723193626
9	0.172921927421379	0.9685683684219815
>10	0.6489933130587038	6.349175898534799
>50	0.021050204733649307	0.8932191876250889
>100	0.00882014681341976	0.995798085907744
>500	6.526728480702201E-4	0.2538320549460686
>1k	1.553967919728292E-4	0.20394597866809633
>5k	3.107935838945837E-5	0.1774275855621951
>10k+	3.107935838945837E-5	0.20787248032670996
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10747	0.17874187474563752	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9173	0.1525634332410657	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.6631792569613615E-5	0.0
5	0.0	0.0	0.0	1.6631792569613615E-5	0.0
6	0.0	0.0	0.0	1.6631792569613615E-5	0.0
7	0.0	0.0	0.0	3.326358513922723E-5	0.0
8	0.0	0.0	0.0	3.326358513922723E-5	0.0
9	0.0	0.0	0.0	3.326358513922723E-5	0.0
10-11	0.0	0.0	0.0	5.821127399364765E-5	0.0
12-13	0.0	0.0	0.0	9.979075541768169E-5	0.0
14-15	8.315896284806807E-6	0.0	0.0	1.3305434055690892E-4	1.6631792569613615E-5
16-17	1.6631792569613615E-5	0.0	0.0	1.5800202941132935E-4	3.326358513922723E-5
18-19	1.6631792569613615E-5	0.0	0.0	2.328450959745906E-4	6.652717027845446E-5
20-21	1.6631792569613615E-5	0.0	0.0	2.9937226625304506E-4	6.652717027845446E-5
22-23	3.326358513922723E-5	0.0	0.0	4.074789179555336E-4	6.652717027845446E-5
24-25	3.326358513922723E-5	0.0	0.0	8.232737321958739E-4	6.652717027845446E-5
26-27	3.326358513922723E-5	0.0	0.0	0.0014053864721323504	8.315896284806807E-5
28-29	3.326358513922723E-5	0.0	0.0	0.003010354455100064	8.315896284806807E-5
30-31	3.326358513922723E-5	0.0	0.0	0.008241053218243546	9.147485913287488E-5
32-33	3.326358513922723E-5	0.0	0.0	0.02141343293337753	1.164225479872953E-4
34-35	3.326358513922723E-5	0.0	0.0	0.044140777479754534	1.164225479872953E-4
36-37	3.326358513922723E-5	0.0	0.0	0.08240221628615066	1.164225479872953E-4
38-39	3.326358513922723E-5	0.0	0.0	0.13929126277051404	1.164225479872953E-4
40-41	3.326358513922723E-5	0.0	0.0	0.2019515412765333	1.164225479872953E-4
42-43	4.9895377708840844E-5	0.0	0.0	0.2703913677004933	1.164225479872953E-4
44-45	4.9895377708840844E-5	0.0	0.0	0.35928829898507814	1.164225479872953E-4
46-47	4.9895377708840844E-5	0.0	0.0	0.46710389431759836	1.164225479872953E-4
48-49	4.9895377708840844E-5	0.0	0.0	0.5866947987894051	1.164225479872953E-4
50-51	4.9895377708840844E-5	0.0	0.0	0.7118074583943235	1.164225479872953E-4
52-53	4.9895377708840844E-5	0.0	0.0	0.8369034862066723	1.2473844427210212E-4
54-55	4.9895377708840844E-5	0.0	0.0	0.9774670811087618	1.3305434055690892E-4
56-57	4.9895377708840844E-5	0.0	0.0	1.1439097452491702	1.3305434055690892E-4
58-59	4.9895377708840844E-5	0.0	0.0	1.3362980057981755	1.3305434055690892E-4
60-61	4.9895377708840844E-5	0.0	0.0	1.536777633432298	1.3305434055690892E-4
62-63	4.9895377708840844E-5	0.0	0.0	1.741498368171672	1.4968613312652253E-4
64-65	4.9895377708840844E-5	0.0	0.0	1.9580443074280414	1.4968613312652253E-4
66-67	4.9895377708840844E-5	0.0	0.0	2.2035129339629687	1.4968613312652253E-4
68-69	4.9895377708840844E-5	0.0	0.0	2.4744697826108286	1.4968613312652253E-4
70-71	4.9895377708840844E-5	0.0	0.0	2.772328555740039	1.4968613312652253E-4
72-73	4.9895377708840844E-5	0.0	0.0	3.0771061545782086	2.16213303404977E-4
74-75	4.9895377708840844E-5	0.0	0.0	3.384311995131542	2.16213303404977E-4
76-77	4.9895377708840844E-5	0.0	0.0	3.7269518697544366	2.16213303404977E-4
78-79	4.9895377708840844E-5	0.0	0.0	4.104036186789001	2.16213303404977E-4
80-81	4.9895377708840844E-5	0.0	0.0	4.505170075879227	2.328450959745906E-4
82-83	4.9895377708840844E-5	0.0	0.0	4.918553280196974	2.4947688854420425E-4
84-85	4.9895377708840844E-5	0.0	0.0	5.336784652048762	2.5779278482901104E-4
86-87	4.9895377708840844E-5	0.0	0.0	5.773809949504215	2.6610868111381783E-4
88	4.9895377708840844E-5	0.0	0.0	6.127202278023365	2.6610868111381783E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9305	0.0	50.592617	1
GTATCAA	16235	0.0	39.166122	1
CAACGCA	18300	0.0	33.206615	5
ATCAACG	18530	0.0	32.89699	3
TATCAAC	20120	0.0	31.463425	2
AACGCAG	20795	0.0	30.01223	6
TCAACGC	21110	0.0	29.009283	4
GTGGTAT	3540	0.0	26.916656	1
ACGCAGA	22825	0.0	26.62238	7
CGCAGAG	23130	0.0	26.169956	8
GTACTAG	3860	0.0	25.296242	1
GCAGAGT	25755	0.0	23.101217	9
GTACATG	25275	0.0	22.806204	1
TACATGG	25240	0.0	22.362482	2
CTGTGCG	4890	0.0	21.912338	9
TGGTATC	4305	0.0	21.615156	2
TAGGCAT	5040	0.0	21.54064	5
CGTTAAC	1080	0.0	21.401554	1
ACATGGG	25290	0.0	21.353193	3
GGGCACC	4550	0.0	21.173582	94
>>END_MODULE