FastQCFastQC Report
Thu 26 May 2016
ERR580045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8876182
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215800.2431225497629499No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169580.19105061162558407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93490.10532681731852728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140250.047.339451
GTATCAA258850.041.362451
CAACGCA287500.035.6073725
ATCAACG297900.034.3644753
GTACTAG68300.033.0454671
TATCAAC325800.032.5365642
AACGCAG329150.031.8174046
TCAACGC350250.029.4167424
GCATCGT71000.029.3152688
ACGCAGA351800.028.9810987
CGCAGAG358300.028.4027358
GTGGTAT49050.028.1838071
TAGGCAT88650.027.8886135
GGGCACC81550.027.03415794
TAGTACT93000.026.8881454
CTGTGCG92300.026.8800289
GTACATG400450.026.5369821
CTAGGCA97200.025.8230274
GCAGAGT392050.025.5530769
TACATGG410450.025.4941862
Produced by FastQC (version 0.11.5)