FastQCFastQC Report
Thu 26 May 2016
ERR580045_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580045_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8876182
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191590.21584730912457634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158200.17822978393187522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91700.10331018449148519No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA144950.048.5868381
GTATCAA256200.039.71031
CAACGCA293550.032.9835665
GTACTAG70150.032.4371531
ATCAACG301000.032.2140433
TATCAAC327800.030.7220542
AACGCAG337650.029.5519376
TAGGCAT90500.028.2528845
TCAACGC353150.027.6830644
GTGGTAT46500.027.5005261
GGGCACC81800.027.11857894
ACGCAGA361050.026.7384247
CTAGGCA96850.026.6915074
GTACATG397350.026.2311631
CGCAGAG369350.026.073648
CTGTGCG93650.025.8946699
TAGTACT93150.025.8343264
TACATGG403850.025.4256652
ACATGGG399450.024.674513
GCATCGT89100.024.5286168
Produced by FastQC (version 0.11.5)