FastQCFastQC Report
Thu 26 May 2016
ERR580044_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580044_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143959
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44820.14255911098077298No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36050.11466434517752935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41350.047.0810741
GTATCAA72600.040.1584171
CAACGCA86050.033.2644585
ATCAACG88350.032.5038573
TATCAAC90300.032.0182952
TCAACGC92400.031.1823944
AACGCAG93950.030.8557766
GTGGTAT19150.028.7301981
ACGCAGA108100.026.3834677
CGCAGAG112700.025.3061878
TGGTATC21750.023.77632
GTACATG116400.023.5929051
TACATGG118700.022.7337992
GCGTAAT1903.6259007E-722.2746261
GCAGAGT126500.021.7307999
GTACTAG11950.021.642941
ACATGGG122400.021.311393
CATGGGG98950.020.4255434
GAGTACT55050.018.60871712-13
TAATACA12400.018.5735684
Produced by FastQC (version 0.11.5)