FastQCFastQC Report
Thu 26 May 2016
ERR580044_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580044_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3143959
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40210.12789606989149666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33720.10725330705648516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39550.046.4816251
GTATCAA72100.040.6259771
CAACGCA83050.033.5027965
ATCAACG84400.033.02473
TATCAAC87300.032.852892
TCAACGC90500.030.9535564
AACGCAG92500.030.6365646
ACGCAGA103500.026.6539927
GTGGTAT18450.025.9929071
CGCAGAG107250.025.6782138
GTACTAG11700.024.914771
GTACATG115100.024.672471
TACATGG117200.023.6290172
ACATGGG120450.022.6332993
GCAGAGT121950.022.2350359
TGGTATC20450.021.8417912
TATATCG1954.679332E-721.6923665
CATGGGG94900.020.6529794
ATGGGGG62350.019.1468055
AGAGTAC110150.018.96389610-11
Produced by FastQC (version 0.11.5)