FastQCFastQC Report
Thu 26 May 2016
ERR580043_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580043_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632270
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145590.25849257936853165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115590.20522808743188803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61840.10979587271206814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110250.047.221981
GTATCAA195350.040.9270741
CAACGCA225600.034.081535
ATCAACG230950.033.3557783
TATCAAC245400.032.3501972
AACGCAG252450.031.2054676
TCAACGC261150.029.7688374
ACGCAGA272900.028.143167
CGCAGAG277950.027.4776868
GTGGTAT41150.027.3151281
GTACTAG42600.027.0477811
GCAGAGT297800.025.0931029
TACCCCG5750.023.7175925
GGGCTAT59000.023.6611846
GCTATTG56600.023.58288
AGGGCTA58050.023.4118655
CTATTGA58900.023.3794469
GCATCGT46550.023.0201768
GAGTACT126800.022.85161612-13
GGCTATT62250.022.6519837
Produced by FastQC (version 0.11.5)