FastQCFastQC Report
Thu 26 May 2016
ERR580043_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580043_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5632270
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120780.21444284453692739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99620.17687362289094805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59320.10532165538939006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105100.047.0981981
GTATCAA191150.040.995851
CAACGCA224600.033.5857625
ATCAACG230750.032.7319453
TATCAAC242600.032.2382742
AACGCAG255600.030.3760596
TCAACGC260950.029.1056864
ACGCAGA273100.027.620517
TACCCCG5000.027.2595025
CGCAGAG279500.026.9546668
TATTCTC30750.026.5946375
GTACTAG40650.026.01961
GTGGTAT38950.024.8621351
AGGGCTA60750.024.756755
GCAGAGT301000.024.7016569
GCTATTG60850.024.0977368
GGGCTAT62400.023.9510616
CTATTGA62150.023.593899
ATTTAGG64800.023.2867371
TAGGCAT56700.023.043675
Produced by FastQC (version 0.11.5)