FastQCFastQC Report
Thu 26 May 2016
ERR580042_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580042_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3234486
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44770.13841457344381766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37790.1168346377136893No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41750.047.3003921
GTATCAA76050.042.041831
CAACGCA87800.034.9117165
ATCAACG89650.034.2993553
TATCAAC94900.032.9974142
TCAACGC94700.032.568084
AACGCAG98350.031.6754066
ACGCAGA107950.028.249137
CGCAGAG112300.027.1548868
GTACTAG12400.025.405341
GCAGAGT124300.023.9707589
GTACATG119100.023.7265531
TACATGG120650.022.8761372
ACATGGG121400.022.036983
GAGTACT53650.020.11150412-13
GTGGTAT21050.020.1030671
CATGGGG93000.019.9688224
CGTTAAC4300.019.6823041
CAGAGTA119200.019.61752910-11
AGAGTAC112000.019.59865610-11
Produced by FastQC (version 0.11.5)