FastQCFastQC Report
Thu 26 May 2016
ERR580042_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580042_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3234486
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40210.12431650654849023No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34880.10783784502390797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41200.044.0608631
GTATCAA70900.040.1014941
CAACGCA84000.032.6166735
ATCAACG85650.031.9344463
TATCAAC89100.031.4368522
AACGCAG93300.030.1704716
TCAACGC91500.029.9945344
ACGCAGA103850.026.4719727
CGCAGAG107650.025.2319458
GTACATG115700.024.3293481
TACATGG119950.023.3124122
GTACTAG15300.022.8049831
ACATGGG121300.022.0840053
GTGGTAT21600.021.6108061
GCAGAGT122800.021.4684859
CATGGGG87600.020.0640144
AGAGTAC110150.019.39157910-11
TAGGCAT21850.019.142075
GAGTACT54550.018.99667512-13
GCATCGT16100.018.3887258
Produced by FastQC (version 0.11.5)