FastQCFastQC Report
Thu 26 May 2016
ERR580041_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580041_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16950883
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT400720.236400664201387No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318840.18809639592226554No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198900.11733902003807116No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA239400.044.6941641
GTATCAA423200.040.2417071
CAACGCA467650.034.5525055
ATCAACG483800.033.5097773
GTACTAG135050.032.1440541
TATCAAC534700.031.6924152
AACGCAG537200.031.0349256
GCATCGT128700.029.5810818
TCAACGC563100.029.0738854
GTACATG604600.028.4012341
TAACGCC62950.028.1663254
ACGCAGA575100.028.1063277
TCTAACG62650.028.0770682
CTAACGC62950.028.0176453
GGGCACC153750.027.48175494
TACATGG615050.027.3915352
CGCAGAG591150.027.3109388
CTGTGCG178450.026.9440469
ACATGGG607550.026.846843
TAGGCAT167550.026.6921445
Produced by FastQC (version 0.11.5)