FastQCFastQC Report
Thu 26 May 2016
ERR580041_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580041_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16950883
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT344500.20323425039273768No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285890.16865788053637087No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185770.10959311087215928No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA246000.044.2979551
GTATCAA420100.037.533211
CAACGCA464300.032.4839445
ATCAACG478300.031.5339643
GTACTAG128700.031.4141461
TATCAAC531200.029.4742322
AACGCAG538150.028.8548646
GTACATG580300.027.9817661
TCAACGC553400.027.491684
GGGCACC151800.027.34312694
TACATGG590050.027.0762332
ACATGGG577700.026.5963363
CTGTGCG174750.026.5182389
ACGCAGA577000.026.1545267
TAGGCAT162300.025.802215
CGCAGAG592150.025.4616348
CTAACGC56900.025.4417933
TAGTACT188200.025.1732124
TAACGCC58900.024.8964274
TCTAACG60050.024.8908672
Produced by FastQC (version 0.11.5)