FastQCFastQC Report
Thu 26 May 2016
ERR580040_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580040_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7827077
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186480.2382498600690909No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139180.17781861606829727No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91000.11626306985353536No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109200.045.3901821
GTATCAA190600.040.9324231
CAACGCA214400.034.870935
ATCAACG220950.033.8625873
TATCAAC240950.032.0671352
AACGCAG245250.031.4684146
TCAACGC252800.029.8557534
GTACTAG50800.028.8825021
GTACATG278400.028.5470621
ACGCAGA261800.028.5275827
TACATGG281650.027.6002242
CGCAGAG270300.027.59438
ACATGGG275550.027.1015433
CTGTGCG68650.026.5628179
TAGTACT71300.025.7220254
CTAACGC23650.025.6508923
CATGGGG224300.025.0115854
TCTAACG24700.024.751182
GCATCGT52000.024.5840848
CTAGTAC77800.024.3595563
Produced by FastQC (version 0.11.5)