FastQCFastQC Report
Thu 26 May 2016
ERR580040_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580040_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7827077
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163030.20828976129914142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127680.16312603031757578No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86620.11066711110673882No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101700.046.3610651
GTATCAA186700.041.066061
CAACGCA217600.033.75975
ATCAACG221550.033.158013
GTACTAG47400.031.7354771
TATCAAC242350.031.321852
AACGCAG244850.030.7505266
TCAACGC249500.029.6317064
GTACATG278050.027.4221381
ACGCAGA268050.027.3873377
TACATGG281950.026.5225942
CGCAGAG277350.026.418168
ACATGGG278000.025.7318573
CTGTGCG68050.024.5870539
TAGGCAT64550.024.5376035
CATGGGG223000.023.9215584
TAGTACT73000.023.435654
GCAGAGT313000.023.1238949
GGGCACC67350.022.7531994
CTAGGCA72650.022.7075354
Produced by FastQC (version 0.11.5)