FastQCFastQC Report
Thu 26 May 2016
ERR580039_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580039_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7774414
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142490.18328069485365714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109280.14056364891295986No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110950.048.8188971
GTATCAA203650.042.2733541
CAACGCA234350.035.587575
ATCAACG238150.035.0231063
TATCAAC249950.033.991742
AACGCAG261700.032.5373736
TCAACGC260600.032.1137924
ACGCAGA288800.028.8323867
CGCAGAG300550.027.6739148
GTACTAG40050.026.4145261
GCAGAGT330400.024.5665749
GTGGTAT48350.024.1167071
GTACATG320200.024.0962831
TACATGG327400.023.265092
ACATGGG330500.022.3773613
GCATCGT38950.022.0778968
GAGTACT136900.021.90943112-13
TAGGCAT53850.021.6508875
CATGGGG256100.020.6716374
CAGAGTA322100.020.24747510-11
Produced by FastQC (version 0.11.5)