FastQCFastQC Report
Thu 26 May 2016
ERR580039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR580039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7774414
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123350.15866147596461933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101110.13005481827955137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA114450.048.9897271
GTATCAA203300.040.4759451
CAACGCA234050.034.115735
ATCAACG238500.033.401233
TATCAAC250250.032.470852
AACGCAG258900.031.3299126
TCAACGC259350.030.8422534
GTGGTAT48200.028.5676671
ACGCAGA291050.027.2232767
CGCAGAG302050.026.0142128
GTACTAG39100.025.0856591
GTACATG328000.023.7506311
TACATGG332700.023.0536732
GCAGAGT336500.022.8621379
TGGTATC58400.022.8548452
ACATGGG334000.021.9934673
GGGCTAT51350.020.8662726
GAGTACT137400.020.5390112-13
CATGGGG258850.020.1896764
TAGGCAT51050.019.7009895
Produced by FastQC (version 0.11.5)