##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR580039_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7774414
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.54167323736554	34.0	31.0	34.0	31.0	34.0
2	32.74328045817987	34.0	31.0	34.0	31.0	34.0
3	32.82410892962479	34.0	31.0	34.0	31.0	34.0
4	36.28997491000608	37.0	37.0	37.0	35.0	37.0
5	36.22226691297891	37.0	37.0	37.0	35.0	37.0
6	36.24921132319426	37.0	37.0	37.0	35.0	37.0
7	36.21928289386184	37.0	37.0	37.0	35.0	37.0
8	36.20964036646363	37.0	37.0	37.0	35.0	37.0
9	37.99700671973476	39.0	38.0	39.0	35.0	39.0
10-11	37.949842328952386	39.0	38.0	39.0	35.0	39.0
12-13	37.91267451154518	39.0	38.0	39.0	35.0	39.0
14-15	39.318750004823514	41.0	39.0	41.0	36.0	41.0
16-17	39.30684229062152	41.0	39.0	41.0	36.0	41.0
18-19	39.30562354924757	41.0	39.0	41.0	36.0	41.0
20-21	39.287614925060595	41.0	39.0	41.0	36.0	41.0
22-23	39.21921993091698	41.0	39.0	41.0	36.0	41.0
24-25	39.13953643322827	40.5	39.0	41.0	35.5	41.0
26-27	39.00533339233028	40.0	38.5	41.0	35.0	41.0
28-29	38.90330216528217	40.0	38.0	41.0	35.0	41.0
30-31	38.80108925251472	40.0	38.0	41.0	35.0	41.0
32-33	38.66864589408282	40.0	38.0	41.0	35.0	41.0
34-35	38.64781551381236	40.0	38.0	41.0	34.5	41.0
36-37	38.70371188104981	40.0	38.0	41.0	35.0	41.0
38-39	38.62088145807517	40.0	38.0	41.0	35.0	41.0
40-41	38.50254366438422	40.0	38.0	41.0	34.0	41.0
42-43	38.48170048572149	40.0	38.0	41.0	34.0	41.0
44-45	38.40005780499983	40.0	38.0	41.0	34.0	41.0
46-47	38.21880684512042	40.0	38.0	41.0	34.0	41.0
48-49	38.020291047016535	40.0	37.0	41.0	33.0	41.0
50-51	37.85705456385523	40.0	37.0	41.0	33.0	41.0
52-53	37.68245632403934	40.0	36.5	41.0	33.0	41.0
54-55	37.48274230057725	40.0	36.0	41.0	33.0	41.0
56-57	37.267883277119026	39.0	35.5	41.0	32.0	41.0
58-59	37.033768847401234	39.0	35.0	41.0	32.0	41.0
60-61	36.77108287518519	38.5	35.0	40.5	32.0	41.0
62-63	36.47927824527997	38.0	35.0	40.0	31.0	41.0
64-65	36.18440740356765	37.0	35.0	40.0	31.0	41.0
66-67	35.850954232692004	37.0	35.0	40.0	31.0	41.0
68-69	35.495619798482565	36.0	35.0	39.0	30.5	41.0
70-71	35.16497391314638	36.0	34.0	39.0	30.0	40.5
72-73	34.809265688706574	35.0	34.0	38.5	30.0	40.0
74-75	34.454072358379676	35.0	34.0	37.0	29.5	39.5
76-77	33.22635268716073	34.5	32.5	36.0	28.0	39.0
78-79	33.852331635541915	35.0	34.0	36.0	29.0	39.0
80-81	33.78811046080129	35.0	34.0	36.0	29.0	37.5
82-83	33.584116436299894	35.0	34.0	35.5	29.0	37.0
84-85	33.37132991631266	35.0	34.0	35.0	29.0	37.0
86-87	33.205834742014	35.0	34.0	35.0	29.0	36.0
88-89	33.054019698462156	35.0	33.5	35.0	29.0	36.0
90-91	32.903720460474574	35.0	33.0	35.0	29.0	36.0
92-93	32.793490416126545	35.0	33.0	35.0	29.0	35.0
94-95	32.70114808653102	35.0	33.0	35.0	29.0	35.0
96-97	32.608463866215516	35.0	33.0	35.0	28.0	35.0
98-99	32.54106740392266	35.0	33.0	35.0	27.0	35.0
100	31.265738742495575	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.16443959145609455
1101	2	0.06314389693838507
1101	3	0.029417128501989964
1101	4	0.054731239405398924
1101	5	-0.014825916629675362
1101	6	0.029609391656748585
1101	7	0.005897483957546967
1101	8	0.012185139443111836
1101	9	0.01212571393097761
1101	10-11	0.02808628113819367
1101	12-13	-0.00442284716611141
1101	14-15	0.05759516338911652
1101	16-17	0.019721067034168982
1101	18-19	0.0022042346879800334
1101	20-21	-0.033223965826074675
1101	22-23	-0.0560683136322524
1101	24-25	-0.07279757911913265
1101	26-27	-0.05997268436459535
1101	28-29	-0.06459831761967649
1101	30-31	-0.07172801235363124
1101	32-33	-0.05105736830703478
1101	34-35	-0.1405457936233958
1101	36-37	-0.025556736789361878
1101	38-39	-0.014228535261814557
1101	40-41	0.006263941723290145
1101	42-43	-0.04902650827544619
1101	44-45	-0.13248009452846077
1101	46-47	-0.024021052522257946
1101	48-49	-0.06480738588348345
1101	50-51	-0.03864147432272347
1101	52-53	-0.10445376254609329
1101	54-55	-0.10589634985539931
1101	56-57	-0.1186415667088454
1101	58-59	-0.0795838074104438
1101	60-61	-0.13910454234930114
1101	62-63	-0.08255157005744707
1101	64-65	-0.07197857243798467
1101	66-67	-0.13864934962910525
1101	68-69	-0.11802307841424664
1101	70-71	-0.18073339377195907
1101	72-73	-0.07798360435750595
1101	74-75	-0.08433780736829988
1101	76-77	-0.12619132448607218
1101	78-79	-0.08821662861230806
1101	80-81	-0.08473883365956425
1101	82-83	-0.11138205294534487
1101	84-85	-0.025790535376309265
1101	86-87	-0.059564168280466845
1101	88-89	-0.055445739714606646
1101	90-91	-0.06955915875324337
1101	92-93	-0.16169341839185591
1101	94-95	-0.19827911013855726
1101	96-97	-0.15570123731642127
1101	98-99	-0.1781099619821589
1101	100	-0.0662206992669212
1102	1	0.09511285966173944
1102	2	0.012129968871491315
1102	3	0.02027214365894281
1102	4	-0.011392490274438671
1102	5	-0.029934987423374082
1102	6	0.006785922816042955
1102	7	-0.009868122366725629
1102	8	0.020053529225656064
1102	9	-0.00723482474971604
1102	10-11	0.008886798779087712
1102	12-13	-0.01960998569580852
1102	14-15	0.012833402099794
1102	16-17	-0.046157578351106565
1102	18-19	-0.02451865828826527
1102	20-21	-0.06761073287871966
1102	22-23	-0.05837735328331206
1102	24-25	-0.037595055382645626
1102	26-27	-0.0938362173173033
1102	28-29	-0.09490056026196214
1102	30-31	-0.06169502528408799
1102	32-33	-0.09063700399948971
1102	34-35	-0.10041495781449328
1102	36-37	-0.14361315613266612
1102	38-39	-0.1631701880011036
1102	40-41	-0.11641410545557562
1102	42-43	-0.09325251409507018
1102	44-45	-0.1035260672328775
1102	46-47	-0.09574470904221499
1102	48-49	-0.12487159273923254
1102	50-51	-0.09476198563699967
1102	52-53	-0.09197011117564813
1102	54-55	-0.1541531729909451
1102	56-57	-0.1614064131272599
1102	58-59	-0.1535633161807013
1102	60-61	-0.1548295963255839
1102	62-63	-0.12509443418971955
1102	64-65	-0.21801126412663052
1102	66-67	-0.23289772420184107
1102	68-69	-0.20840035215735497
1102	70-71	-0.1375850702542536
1102	72-73	-0.17101662652262206
1102	74-75	-0.21085883507620196
1102	76-77	-0.13404169551407108
1102	78-79	-0.08998468337593835
1102	80-81	-0.11894477979010531
1102	82-83	-0.10651818289007764
1102	84-85	-0.13869699328981966
1102	86-87	-0.1545682099545047
1102	88-89	-0.062267718138777184
1102	90-91	-0.12784520117912024
1102	92-93	-0.12909761322612923
1102	94-95	-0.1654036610481029
1102	96-97	-0.2027900976124073
1102	98-99	-0.09638208831034234
1102	100	0.004176227992186199
1103	1	0.09849693146025373
1103	2	0.06655442945947954
1103	3	0.04527905829811374
1103	4	0.02145395014693463
1103	5	0.013540985771093972
1103	6	0.04078710437060096
1103	7	0.030543840184357407
1103	8	0.0031308609432514345
1103	9	0.008348246956309424
1103	10-11	-0.032527146986886635
1103	12-13	0.01711103411609116
1103	14-15	0.052033540759815367
1103	16-17	0.021823568157728346
1103	18-19	0.016155103960052486
1103	20-21	-0.02245229771573065
1103	22-23	-0.05772800183211757
1103	24-25	-0.06644897424667562
1103	26-27	-0.014509946850687072
1103	28-29	-0.08023430067176918
1103	30-31	-0.06815537582818365
1103	32-33	-0.0897426593492412
1103	34-35	-0.04543712563369695
1103	36-37	-0.03586092310953859
1103	38-39	0.01194457448210784
1103	40-41	0.0156670732021027
1103	42-43	-0.06783720796494919
1103	44-45	-0.0846719746910054
1103	46-47	-0.030772029945445922
1103	48-49	0.0010210740676228625
1103	50-51	0.011102980276199048
1103	52-53	-9.545647662179135E-5
1103	54-55	-0.046340393275215774
1103	56-57	-0.03285109505970496
1103	58-59	-0.018499344923533556
1103	60-61	-4.477180797692881E-4
1103	62-63	-0.04429077150210503
1103	64-65	-0.017959314727605147
1103	66-67	-0.05748683539034971
1103	68-69	0.010810932995632072
1103	70-71	0.0020076268283517607
1103	72-73	-6.42948308396285E-4
1103	74-75	-0.08496469288972719
1103	76-77	-0.11713726901761845
1103	78-79	-0.016260325052606106
1103	80-81	0.02448670269073716
1103	82-83	0.037408497927721385
1103	84-85	0.08062665324624163
1103	86-87	0.06493452681853995
1103	88-89	0.0564595110006465
1103	90-91	-0.01317290856925979
1103	92-93	0.02113232228069961
1103	94-95	0.09950984891158043
1103	96-97	0.09945847873847669
1103	98-99	0.021888663188541102
1103	100	0.07270626382643286
1104	1	0.06979191989884015
1104	2	0.04972893311707338
1104	3	0.02364406433579802
1104	4	0.015853170131919114
1104	5	-0.023132788216109645
1104	6	0.009131170931965471
1104	7	-0.0250205798221117
1104	8	0.02916934438268015
1104	9	0.01047813226968941
1104	10-11	5.682539302185319E-4
1104	12-13	-0.008778080846404634
1104	14-15	0.05011362393761942
1104	16-17	0.02371942824843387
1104	18-19	-0.06413336485727683
1104	20-21	-0.07347129432611155
1104	22-23	-0.09412587642507475
1104	24-25	-0.0809689289030544
1104	26-27	-0.06621972784438412
1104	28-29	-0.11967964280567145
1104	30-31	-0.1450837352164669
1104	32-33	-0.13646484800660375
1104	34-35	-0.11716320687580861
1104	36-37	-0.0728210749944509
1104	38-39	-0.06965106971158974
1104	40-41	-0.07009808305668486
1104	42-43	-0.0684922857521002
1104	44-45	-0.08225077104484058
1104	46-47	-0.06911892211693527
1104	48-49	-0.023210246027794312
1104	50-51	-0.045254355656332734
1104	52-53	-0.084410746437932
1104	54-55	-0.1391073014760309
1104	56-57	-0.06134362807018334
1104	58-59	-0.0816321927449053
1104	60-61	-0.08867192809225344
1104	62-63	-0.060353552320023596
1104	64-65	-0.0638668654814083
1104	66-67	-0.034309230414464764
1104	68-69	-7.25214016419784E-4
1104	70-71	-0.053104400724613754
1104	72-73	-0.05210229705387093
1104	74-75	-0.1842622108310792
1104	76-77	-0.23161770816174254
1104	78-79	-0.06003508201109753
1104	80-81	-0.05289584205088005
1104	82-83	0.014337869308356233
1104	84-85	-0.012190017885409077
1104	86-87	0.0031970969189316634
1104	88-89	0.04970145000348225
1104	90-91	0.014687089381261842
1104	92-93	0.006337065863355917
1104	94-95	0.012268140037399178
1104	96-97	-0.04790348032571501
1104	98-99	-0.019328852853128353
1104	100	-0.0037543638542523183
1105	1	0.07117141151062611
1105	2	0.029050426418756103
1105	3	-0.020454356061648582
1105	4	0.017878144493209902
1105	5	-0.013741925271951061
1105	6	0.008295735976886931
1105	7	0.02168750653035545
1105	8	-0.0179612163149514
1105	9	-0.02303649953262976
1105	10-11	-0.04968579505100479
1105	12-13	-0.022932583906928983
1105	14-15	0.02395125511429086
1105	16-17	-0.003661746810749378
1105	18-19	-0.04318331357985272
1105	20-21	-0.062127293784314475
1105	22-23	-0.09783687817301967
1105	24-25	-0.07869184055753209
1105	26-27	-0.0784519726344115
1105	28-29	-0.08926286057667454
1105	30-31	-0.13252891030145264
1105	32-33	-0.1455117652338842
1105	34-35	-0.1144733191701306
1105	36-37	-0.08637347148823693
1105	38-39	-0.06051756136852049
1105	40-41	-0.053707126587674736
1105	42-43	0.002110111064183684
1105	44-45	0.022484650365257153
1105	46-47	0.007215903314083505
1105	48-49	-0.031012395809462134
1105	50-51	-0.042478038084631464
1105	52-53	-0.008839116073957598
1105	54-55	-0.011687748976342505
1105	56-57	-0.11426523166669966
1105	58-59	-0.06247951180656486
1105	60-61	-0.056927415421242245
1105	62-63	-0.020977492102915107
1105	64-65	0.04651237780576878
1105	66-67	-0.04004939901720661
1105	68-69	0.010384732513166739
1105	70-71	-0.0904238010045404
1105	72-73	-0.07863086405958342
1105	74-75	-0.07615949151529833
1105	76-77	-0.04277594899146209
1105	78-79	-0.033627855442297516
1105	80-81	-0.04754468847016824
1105	82-83	-0.0174484876727945
1105	84-85	0.05613617745898836
1105	86-87	-0.004044629356386054
1105	88-89	0.021772590168218642
1105	90-91	-0.0017133808230127556
1105	92-93	0.005700854974485026
1105	94-95	-0.03914408527364799
1105	96-97	-0.07945667016826974
1105	98-99	-0.08256221671641839
1105	100	0.04666972298841188
1106	1	0.13584837135778827
1106	2	0.10265923773285124
1106	3	0.022573639631325193
1106	4	0.013799327918128768
1106	5	6.558204108131349E-5
1106	6	0.04938240137678207
1106	7	0.018919380359719185
1106	8	0.04862721416568405
1106	9	0.03812677980771184
1106	10-11	0.05648919224894655
1106	12-13	0.015217332266281858
1106	14-15	0.06632894684626933
1106	16-17	0.027694709015698038
1106	18-19	-0.0067916595486039455
1106	20-21	0.014939049596598863
1106	22-23	0.015498303615430586
1106	24-25	0.022594920108893746
1106	26-27	-0.03960600969916328
1106	28-29	-0.019029628843746593
1106	30-31	0.0320245876090226
1106	32-33	5.307634527156324E-4
1106	34-35	0.06038783664429559
1106	36-37	0.033502914691524666
1106	38-39	0.07038518274712402
1106	40-41	0.038310730393611436
1106	42-43	0.09119003929758662
1106	44-45	0.10881057436660768
1106	46-47	0.08785466089371852
1106	48-49	0.06126511741087626
1106	50-51	0.07672122214553667
1106	52-53	0.08525985253979229
1106	54-55	0.05448759056418595
1106	56-57	0.08014294923372489
1106	58-59	0.06979676049285644
1106	60-61	0.039220252229114294
1106	62-63	-0.005509315907659129
1106	64-65	0.018822019244254307
1106	66-67	0.0047373758681033
1106	68-69	0.11073719929590453
1106	70-71	0.08277833402575396
1106	72-73	0.05479510208136418
1106	74-75	0.028398970078853836
1106	76-77	0.02333303036400025
1106	78-79	0.07564835157950967
1106	80-81	0.09642076804007615
1106	82-83	0.11327629416426532
1106	84-85	0.17462476809597405
1106	86-87	0.12750365216203363
1106	88-89	0.12351441980269584
1106	90-91	0.13224210954110305
1106	92-93	0.09964255948276701
1106	94-95	0.0270256313439603
1106	96-97	0.1684859540433905
1106	98-99	0.1346622665240389
1106	100	0.22278664876195364
1107	1	0.1270454078968868
1107	2	0.07414666148093829
1107	3	0.00481371915448392
1107	4	0.03668634106136892
1107	5	0.04367577570025105
1107	6	0.01774219370420127
1107	7	0.019338845412896433
1107	8	5.041166277308662E-4
1107	9	-0.019585094679946735
1107	10-11	-0.006527313851549366
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2316	9	-0.016533419430800222
2316	10-11	0.021261190862190915
2316	12-13	0.0280593803670115
2316	14-15	0.03427329873817797
2316	16-17	0.011197091348705612
2316	18-19	0.011244580138942695
2316	20-21	0.0016866511563904396
2316	22-23	-0.031412026781787006
2316	24-25	0.029748055185486066
2316	26-27	0.08206171544127727
2316	28-29	0.0839798115150856
2316	30-31	-0.22392280777848583
2316	32-33	-0.06273068570526164
2316	34-35	-0.1550913451708169
2316	36-37	-0.402381187032411
2316	38-39	-0.2584611422012628
2316	40-41	-0.23607021585126375
2316	42-43	-0.30626993045153483
2316	44-45	-0.15977484446685963
2316	46-47	-0.34797575013158877
2316	48-49	-0.3490859890777287
2316	50-51	-0.2658553484607964
2316	52-53	-0.29299035225187
2316	54-55	-0.17964143892274365
2316	56-57	-0.9398606710533883
2316	58-59	-0.7942215741137133
2316	60-61	-0.42182254385974716
2316	62-63	-0.20189761931226258
2316	64-65	-0.07151028015962879
2316	66-67	-0.002376641699484594
2316	68-69	-0.12223901419058336
2316	70-71	-0.13285655081536873
2316	72-73	-0.02944560405870078
2316	74-75	-0.02397093735838496
2316	76-77	-0.06266489564100652
2316	78-79	-0.08318637617797009
2316	80-81	-0.10192034277445572
2316	82-83	-0.2252320555063818
2316	84-85	-0.08429479096532333
2316	86-87	-0.14150669020054352
2316	88-89	-0.13980164046587618
2316	90-91	-0.09185059435315424
2316	92-93	-0.1618173366333977
2316	94-95	-0.1452854617679833
2316	96-97	-0.34852862835035836
2316	98-99	-0.13722666429472952
2316	100	-0.4115536152735615
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	10.0
13	48.0
14	254.0
15	678.0
16	1681.0
17	3586.0
18	6533.0
19	10376.0
20	16106.0
21	24300.0
22	33337.0
23	36837.0
24	36957.0
25	39322.0
26	46232.0
27	57542.0
28	71993.0
29	89407.0
30	110612.0
31	138288.0
32	175862.0
33	231128.0
34	344000.0
35	529748.0
36	951708.0
37	2000628.0
38	2364683.0
39	452503.0
40	53.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84716852781001	19.613555130159558	13.903095767022974	23.636180575007455
2	15.797575210010747	20.526360741534614	39.19080711939909	24.485256929055545
3	18.87397085020472	25.654271527169737	30.012835953326473	25.458921669299073
4	13.3653567005127	17.32788822898148	38.479209057817634	30.827546012688178
5	14.086885851743219	37.72686244563476	33.97124712134838	14.215004581273647
6	30.54430854852854	36.81321833388343	18.404062351194572	14.238410766393455
7	27.752984890037276	32.442680194129274	21.856496579096	17.94783833673745
8	26.983271101234813	33.956704775975375	19.982105350759948	19.07791877202986
9	26.564210389831565	16.163342285190794	20.46199611071421	36.81045121426342
10-11	25.427558195898992	25.74006424539381	27.73189397857162	21.10048358013557
12-13	26.24181770706716	23.245558183836458	27.697407263059194	22.815216846037192
14-15	23.72169305275157	24.764880403226257	25.32901436890037	26.184412175121807
16-17	23.152128446116254	27.581066021266707	25.972402514713664	23.294403017903367
18-19	22.787592164700506	27.111457007321338	27.760078859533806	22.340871968444354
20-21	23.98898074956191	26.146769372199984	27.45404802907802	22.410201849160085
22-23	23.88768293689984	25.873379071470705	27.198014715472652	23.040923276156803
24-25	23.555730374019188	26.05654265389006	27.41782853344316	22.969898438647597
26-27	23.37060418082128	26.369242508163477	27.05833042416116	23.201822886854085
28-29	23.09634795016452	26.432376967065903	27.333613900542204	23.137661182227372
30-31	23.70634307645199	26.07641462186205	27.28504781903264	22.93219448265332
32-33	23.64990998049095	26.3008188912967	26.899527421698465	23.14974370651389
34-35	23.711391264877296	26.38557539069834	27.13890573912287	22.76412760530149
36-37	23.629008842838452	26.19978570534498	26.959523164161308	23.21168228765526
38-39	23.65203945244108	26.226565947248375	27.148966798354905	22.972427801955643
40-41	23.658438328695997	25.766533846962037	27.4042576019164	23.170770222425567
42-43	23.44828446733125	25.873942231401642	27.1439398850926	23.533833416174506
44-45	23.50629190198472	26.19735093178907	27.053953346521624	23.24240381970458
46-47	23.50517207779052	26.046433250514056	26.64688051320081	23.801514158494612
48-49	23.80944631682228	25.947812083776324	26.89045107028279	23.352290529118605
50-51	23.682974984599564	26.149990269008843	26.650309854567666	23.516724891823923
52-53	23.43614602057034	26.26535422521818	26.599302453273648	23.69919730093783
54-55	23.408455354977328	26.117608877235792	26.707762217284248	23.76617355050263
56-57	23.77451604612987	26.049926952543462	26.436336292533518	23.739220708793145
58-59	23.319775752933854	26.240069695666072	26.87743311468972	23.562721436710348
60-61	23.507384286117716	26.374352271541124	26.588735912784305	23.529527529556855
62-63	23.563421091287772	26.1294080602104	26.47928350941263	23.827887339089195
64-65	23.147288785020702	26.067610377080058	26.71826647090409	24.066834366995145
66-67	23.449233259682615	26.015037352201464	26.57042443973306	23.96530494838286
68-69	23.13864589010445	26.073975019915046	26.716806831970015	24.070572258010493
70-71	23.211039691045475	25.829580821439947	26.710664354104036	24.248715133410546
72-73	23.308049296860762	26.021194187394293	26.613241710697956	24.057514805047
74-75	23.2919271836636	26.222384019151495	26.517471174026102	23.9682176231588
76-77	23.123333453604126	26.12501589407475	26.348089273343806	24.40356137897732
78-79	23.003830061350953	26.065769624176514	26.53855246937083	24.3918478451017
80-81	22.981342291996757	26.38878062722478	26.49437755374259	24.135499527035876
82-83	23.3132323076931	25.984494486774278	26.857121762256515	23.84515144327611
84-85	23.365179315984104	26.043159165727353	26.43489253620876	24.156768982079786
86-87	22.74606333966228	26.36171102384523	26.695705936806206	24.196519699686284
88-89	22.93481474497403	25.808322335017706	26.72903586839015	24.52782705161811
90-91	23.048975342412188	25.78005999689751	26.748816179045026	24.422148481645277
92-93	23.034631108609403	25.935064114841644	26.669983598424185	24.360321178124767
94-95	22.582167158091202	25.85357853429322	26.853248273850543	24.711006033765027
96-97	22.582799494872674	25.80181245138214	26.879346665976357	24.736041387768832
98-99	22.397110618702655	25.937473530437206	26.744058389098026	24.921357461762113
100	22.571407035085386	25.696846789021272	26.568238105516244	25.163508070377095
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	154.0
1	129.5
2	163.5
3	236.0
4	186.5
5	176.5
6	359.5
7	715.0
8	1221.0
9	1598.0
10	1526.5
11	1393.0
12	1569.0
13	1867.0
14	2169.0
15	2525.0
16	2961.0
17	3434.5
18	3944.5
19	4465.5
20	4995.5
21	5602.0
22	6399.0
23	7358.5
24	8566.0
25	10167.0
26	12696.5
27	15953.0
28	19660.5
29	25622.0
30	34387.5
31	44861.5
32	59957.0
33	79826.0
34	100242.0
35	122185.5
36	151356.5
37	188118.5
38	214569.5
39	227266.0
40	233857.0
41	237592.0
42	247682.5
43	266697.5
44	287048.0
45	313638.0
46	337889.0
47	354929.0
48	370967.0
49	374488.0
50	364894.5
51	356568.0
52	347429.5
53	334237.0
54	322014.5
55	293924.0
56	259782.5
57	223467.0
58	188801.0
59	163802.5
60	132867.0
61	102886.5
62	79864.0
63	60582.5
64	43495.0
65	29226.5
66	20376.0
67	14827.5
68	11101.5
69	8323.5
70	6184.5
71	4475.5
72	3205.5
73	2354.5
74	1721.5
75	1107.0
76	662.5
77	362.5
78	222.5
79	137.0
80	81.0
81	42.5
82	26.5
83	18.5
84	13.5
85	10.0
86	6.5
87	4.5
88	2.5
89	3.5
90	3.0
91	1.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.352232335453193
2	6.431352896822835E-4
3	0.00201944480960237
4	0.0011705062272217557
5	0.004823514672617126
6	0.0
7	9.003894055551968E-5
8	2.9584223325385036E-4
9	0.0030355985673003777
10-11	5.7882176071405515E-5
12-13	5.145082317458268E-5
14-15	2.5082276297609056E-4
16-17	1.2862705793645668E-4
18-19	0.0010868986395630592
20-21	2.5082276297609056E-4
22-23	1.5435246952374802E-4
24-25	0.0
26-27	0.0019551312806341415
28-29	0.011261298922336785
30-31	0.0033507348592446964
32-33	0.014136113667216592
34-35	0.011132671864400328
36-37	0.012927019322613895
38-39	0.01291415661682025
40-41	0.014078231491145184
42-43	0.006457078308410125
44-45	0.0012283884032931614
46-47	0.005903981959283362
48-49	0.013177842085589989
50-51	0.003640145739601724
52-53	0.005846099783211957
54-55	0.005370179668847066
56-57	2.0580329269833072E-4
58-59	5.46664996229941E-4
60-61	4.309006440871299E-4
62-63	9.775656403170708E-4
64-65	3.923125267061929E-4
66-67	0.0013698781670232636
68-69	0.0018136415169040393
70-71	8.489385823806141E-4
72-73	0.0026625800992846533
74-75	0.007833387828330212
76-77	0.0037366160330540668
78-79	0.0016207009299993545
80-81	5.145082317458267E-4
82-83	2.3152870428562206E-4
84-85	0.0015563874010311258
86-87	0.0044183394401172875
88-89	0.001511367930753366
90-91	1.5435246952374802E-4
92-93	0.0020708956327769525
94-95	0.003170656978133657
96-97	0.0017557593408326338
98-99	0.0019872880451182557
100	0.0023152870428562204
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7774414.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.14106577302125
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.17143254109233	42.54265072077836
2	15.077885803446813	17.532887004326092
3	5.344578917939893	9.322185431911384
4	2.351831407866749	5.469519382873513
5	1.2395559383298367	3.6034551669887054
6	0.7289928094324624	2.543065132876341
7	0.45908110388665124	1.8684025259357493
8	0.32853028775135235	1.5280880854864782
9	0.21619071871183537	1.1312602916527401
>10	1.0178744455240851	10.012723908431143
>50	0.04571110857358113	1.7880417765521086
>100	0.017155906296860556	1.7161744634291658
>500	9.322541727674353E-4	0.3462053207867625
>1k	1.6450464988255087E-4	0.1542271701574349
>5k	2.7417441645186922E-5	0.08330658306936621
>10k+	5.4834883290373844E-5	0.35780703474454534
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12335	0.15866147596461933	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10111	0.13005481827955137	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.2862705793645668E-5	0.0	1.2862705793645668E-5	0.0
3	0.0	1.2862705793645668E-5	0.0	1.2862705793645668E-5	0.0
4	0.0	1.2862705793645668E-5	0.0	6.431352896822834E-5	0.0
5	0.0	1.2862705793645668E-5	0.0	6.431352896822834E-5	0.0
6	0.0	1.2862705793645668E-5	0.0	7.717623476187401E-5	0.0
7	0.0	1.2862705793645668E-5	0.0	9.003894055551968E-5	0.0
8	0.0	1.2862705793645668E-5	0.0	1.1576435214281102E-4	1.2862705793645668E-5
9	0.0	1.2862705793645668E-5	0.0	2.5725411587291337E-4	3.8588117380937005E-5
10-11	0.0	1.2862705793645668E-5	0.0	4.7592011436488975E-4	3.8588117380937005E-5
12-13	0.0	1.2862705793645668E-5	0.0	5.595277020235866E-4	5.1450823174582674E-5
14-15	0.0	1.2862705793645668E-5	0.0	6.302725838886378E-4	8.360758765869685E-5
16-17	0.0	1.2862705793645668E-5	0.0	7.331742302378031E-4	1.4148976373010234E-4
18-19	0.0	1.2862705793645668E-5	0.0	8.232131707933228E-4	1.5435246952374802E-4
20-21	0.0	1.2862705793645668E-5	0.0	9.775656403170708E-4	1.672151753173937E-4
22-23	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.0012476824619836297	2.1223464559515354E-4
24-25	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.0017428966350389883	2.5725411587291337E-4
26-27	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.0023667378660308033	2.7011682166655905E-4
28-29	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.003839517679403232	2.7011682166655905E-4
30-31	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.009081070290313843	2.829795274602047E-4
32-33	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.022464715668602163	3.4729305642843304E-4
34-35	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.04541821415736286	3.601557622220787E-4
36-37	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.0839613120680221	3.601557622220787E-4
38-39	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.14071157002958679	3.6658711511890153E-4
40-41	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.20457490429503755	3.730184680157244E-4
42-43	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.2725800298260422	3.858811738093701E-4
44-45	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.36013003681049144	4.051752324998386E-4
46-47	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.46288376204302983	4.309006440871299E-4
48-49	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.5793812884160787	4.7592011436488975E-4
50-51	1.2862705793645668E-5	1.2862705793645668E-5	0.0	0.7033584782081325	4.8235146726171256E-4
52-53	2.5725411587291337E-5	1.2862705793645668E-5	0.0	0.8307378536826056	5.466649962299409E-4
54-55	2.5725411587291337E-5	1.2862705793645668E-5	0.0	0.9724205579996126	6.23841230991815E-4
56-57	2.5725411587291337E-5	1.2862705793645668E-5	0.0	1.1395521257293475	6.431352896822835E-4
58-59	2.5725411587291337E-5	1.2862705793645668E-5	0.0	1.3255983023286384	6.559979954759291E-4
60-61	2.5725411587291337E-5	1.2862705793645668E-5	0.0	1.5210728422746718	6.688607012695748E-4
62-63	2.5725411587291337E-5	1.2862705793645668E-5	0.0	1.7276350346148277	6.688607012695748E-4
64-65	2.5725411587291337E-5	1.2862705793645668E-5	0.0	1.9395931320354176	6.688607012695748E-4
66-67	2.5725411587291337E-5	1.2862705793645668E-5	0.0	2.1778297373924262	6.752920541663976E-4
68-69	2.5725411587291337E-5	1.2862705793645668E-5	0.0	2.443380298502241	6.881547599600433E-4
70-71	2.5725411587291337E-5	1.2862705793645668E-5	0.0	2.7356209741338704	7.074488186505118E-4
72-73	2.5725411587291337E-5	1.2862705793645668E-5	0.0	3.0313603057413716	7.910564063092087E-4
74-75	2.5725411587291337E-5	1.2862705793645668E-5	0.0	3.331588721670855	7.974877592060315E-4
76-77	2.5725411587291337E-5	1.2862705793645668E-5	0.0	3.6595041118211613	7.974877592060315E-4
78-79	2.5725411587291337E-5	1.2862705793645668E-5	0.0	4.023685900956651	7.974877592060315E-4
80-81	2.5725411587291337E-5	1.2862705793645668E-5	0.0	4.414294119145186	8.296445236901457E-4
82-83	2.5725411587291337E-5	1.2862705793645668E-5	0.0	4.819835938760143	8.93958052658374E-4
84-85	2.5725411587291337E-5	1.2862705793645668E-5	0.0	5.230349194164345	9.003894055551969E-4
86-87	2.5725411587291337E-5	1.2862705793645668E-5	0.0	5.656188363521675	9.132521113488425E-4
88	2.5725411587291337E-5	1.2862705793645668E-5	0.0	5.992580276789994	9.261148171424881E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11445	0.0	48.989727	1
GTATCAA	20330	0.0	40.475945	1
CAACGCA	23405	0.0	34.11573	5
ATCAACG	23850	0.0	33.40123	3
TATCAAC	25025	0.0	32.47085	2
AACGCAG	25890	0.0	31.329912	6
TCAACGC	25935	0.0	30.842253	4
GTGGTAT	4820	0.0	28.567667	1
ACGCAGA	29105	0.0	27.223276	7
CGCAGAG	30205	0.0	26.014212	8
GTACTAG	3910	0.0	25.085659	1
GTACATG	32800	0.0	23.750631	1
TACATGG	33270	0.0	23.053673	2
GCAGAGT	33650	0.0	22.862137	9
TGGTATC	5840	0.0	22.854845	2
ACATGGG	33400	0.0	21.993467	3
GGGCTAT	5135	0.0	20.866272	6
GAGTACT	13740	0.0	20.53901	12-13
CATGGGG	25885	0.0	20.189676	4
TAGGCAT	5105	0.0	19.700989	5
>>END_MODULE